ArticleThe Plant cell2022
Phosphorylation of the plasma membrane H+-ATPase AHA2 by BAK1 is required for ABA-induced stomatal closure in Arabidopsis.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 94 citations in OpenAlex.
- Review
- Receptor-like kinase-mediated cell wall integrity signaling in plant environmental stress responses.Planta · 2026Review
- Water use efficiency in cereal crops: stomatal, hydraulic, biochemical mechanisms, and translational strategies for improvement.Photosynthesis research · 2026Review
- Nitric Oxide Enhances Drought Tolerance inPlants (Basel, Switzerland) · 2026Article
- Transcriptome Dynamics Provide Insight into the Mechanisms Underlying Cucumber Stomatal Movement Regulated by Blue Light (BL) and Drought Stress.International journal of molecular sciences · 2026Article
- GhGLDH35A gene-mediated ROS homeostasis and stomatal movement via the ascorbic acid pathway confers alkaline stress tolerance.Journal of advanced research · 2026Article
- Integration of light quality signals regulates ABA abundance and stomatal movements during seedling establishment.The New phytologist · 2026Article
- Molecular Mechanisms and Experimental Strategies for Understanding Plant Drought Response.Plants (Basel, Switzerland) · 2026Review
- Spatiotemporal dynamics of AHA2 reveal that RALF1 induces its endocytosis and vacuolar degradation.Plant communications · 2025Article
- Exogenously applied ABA alleviates dysplasia of maize (Plant signaling & behavior · 2025Article
- Single-cell transcriptional decoding of iron deficiency responses in maize root tips.Plant cell reports · 2025Article
- Response of hidden architects to salt stress.Planta · 2025Review
- Non-invasive micro-test technology and applications.Biophysics reports · 2025Article
- Identification and Analysis of the Plasma Membrane HPlants (Basel, Switzerland) · 2024Article
- Osmotic signaling releases PP2C-mediated inhibition of Arabidopsis SnRK2s via the receptor-like cytoplasmic kinase BIK1.The EMBO journal · 2024Article
- Zinc Enhances Cadmium Accumulation in Shoots of HyperaccumulatorPlants (Basel, Switzerland) · 2024Article
- Small Peptides: Orchestrators of Plant Growth and Developmental Processes.International journal of molecular sciences · 2024Review
- Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistance.Nature communications · 2024Article
- Apple Glycosyltransferase MdUGT73AR4 Glycosylates ABA to Regulate Stomatal Movement Involved in Drought Stress.International journal of molecular sciences · 2024Article
- The role of CBL-CIPK signaling in plant responses to biotic and abiotic stresses.Plant molecular biology · 2024Review
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stomatal opening is largely promoted by light-activated plasma membrane-localized proton ATPases (PM H+-ATPases), while their closure is mainly modulated by abscisic acid (ABA) signaling during drought stress. It is unknown whether PM H+-ATPases participate in ABA-induced stomatal closure. We established that BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) interacts with, phosphorylates and activates the major PM Arabidopsis H+-ATPase isoform 2 (AHA2). Detached leaves from aha2-6 single mutant Arabidopsis thaliana plants lost as much water as bak1-4 single and aha2-6 bak1-4 double mutants, with all three mutants losing more water than the wild-type (Columbia-0 [Col-0]). In agreement with these observations, aha2-6, bak1-4, and aha2-6 bak1-4 mutants were less sensitive to ABA-induced stomatal closure than Col-0, whereas the aha2-6 mutation did not affect ABA-inhibited stomatal opening under light conditions. ABA-activated BAK1 phosphorylated AHA2 at Ser-944 in its C-terminus and activated AHA2, leading to rapid H+ efflux, cytoplasmic alkalinization, and reactive oxygen species (ROS) accumulation, to initiate ABA signal transduction and stomatal closure. The phosphorylation-mimicking mutation AHA2S944D driven by its own promoter could largely compensate for the defective phenotypes of water loss, cytoplasmic alkalinization, and ROS accumulation in both aha2-6 and bak1-4 mutants. Our results uncover a crucial role of AHA2 in cytoplasmic alkalinization and ABA-induced stomatal closure during the plant's response to drought stress.
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