ArticleeLife2022
Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Tapenade: Spatial Quantification of Mechanical and Genetic Fields in Dense 3D Organoids From Cell to Tissue Scale.Bio-protocol · 2026Article
- A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales.eLife · 2026Article
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- Optogenetic WNT signaling drives germ layer self-organization in a human gastruloid model.bioRxiv : the preprint server for biology · 2026Article
- In vitro approaches to study centriole and cilium function in early mouse embryogenesis.Life science alliance · 2026Article
- Non-cell-autonomous control of mouse gastruloid development by the ultra-conserved lncRNA T-UCstem1.The EMBO journal · 2025Article
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy.Communications biology · 2025Article
- Fine-tuning mechanical constraints reveals uncoupled patterning and gene expression programs in murine gastruloids.Development (Cambridge, England) · 2025Article
- Coping with uncertainty: Challenges for robust pattern formation in dynamical tissues.Seminars in cell & developmental biology · 2025Review
- Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudoembryos.Science advances · 2025Article
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- The Modulation of Cell Plasticity by Budesonide: Beyond the Metabolic and Anti-Inflammatory Actions of Glucocorticoids.Pharmaceutics · 2025Review
- Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation.PloS one · 2025Article
- Three-dimensional stem cell models of mammalian gastrulation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Early autonomous patterning of the anteroposterior axis in gastruloids.Development (Cambridge, England) · 2024Article
- Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking.Nature cell biology · 2024Article
- Non-invasive prediction of human embryonic ploidy using artificial intelligence: a systematic review and meta-analysis.EClinicalMedicine · 2024Article
- The logic of monsters: development and morphological diversity in stem-cell-based embryo models.Interface focus · 2024Review
- Article
- BPA Exposure Affects Mouse Gastruloids Axial Elongation by Perturbing the Wnt/β-Catenin Pathway.International journal of molecular sciences · 2024Article
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Shaping the animal body plan is a complex process that involves the spatial organization and patterning of the different germ layers. Recent advances in live imaging have started to unravel the cellular choreography underlying this process in mammals, however, the sequence of events transforming an unpatterned cell ensemble into structured territories is largely unknown. Here, using gastruloids -3D aggregates of mouse embryonic stem cells- we study the formation of one of the three germ layers, the endoderm. We show that the endoderm is generated from an epiblast-like homogeneous state by a three-step mechanism: (i) a loss of E-cadherin mediated contacts in parts of the aggregate leading to the appearance of islands of E-cadherin expressing cells surrounded by cells devoid of E-cadherin, (ii) a separation of these two populations with islands of E-cadherin expressing cells flowing toward the aggregate tip, and (iii) their differentiation into an endoderm population. During the flow, the islands of E-cadherin expressing cells are surrounded by cells expressing T-Brachyury, reminiscent of the process occurring at the primitive streak. Consistent with recent in vivo observations, the endoderm formation in the gastruloids does not require an epithelial-to-mesenchymal transition, but rather a maintenance of an epithelial state for a subset of cells coupled with fragmentation of E-cadherin contacts in the vicinity, and a sorting process. Our data emphasize the role of signaling and tissue flows in the establishment of the body plan.
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