Evidence map›Paper›PMID 35403421›Full record

ArticleACS applied materials & interfaces2022

Spatial and Temporal Modulation of Cell Instructive Cues in a Filamentous Supramolecular Biomaterial.

Ciqing Tong, Joeri A J Wondergem, Marijn van den Brink, Markus C Kwakernaak, Ying Chen, Marco M R M Hendrix, Ilja K Voets, Erik H J Danen, Sylvia Le Dévédec, Doris Heinrich and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS applied materials & interfaces, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. One- and Two-Dimensional Polymorphism in a Hydrogen-Bonding Bis-Ureido-Squaramide.Angewandte Chemie (International ed. in English) · 2026
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ciqing TongDepartment of Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Joeri A J WondergemBiological and Soft Matter Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, P.O. Box 9504, 2300 RA Leiden, The Netherlands.ORCID 0000-0003-0021-4220
Marijn van den BrinkDepartment of Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Markus C KwakernaakDepartment of Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Ying ChenDepartment of Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Marco M R M HendrixInstitute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MD Eindhoven, The Netherlands.
Ilja K VoetsInstitute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MD Eindhoven, The Netherlands.ORCID 0000-0003-3543-4821
Erik H J DanenDivision of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2333 CC Leiden, The Netherlands.
Sylvia Le DévédecDivision of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2333 CC Leiden, The Netherlands.ORCID 0000-0002-0615-9616
Doris HeinrichBiological and Soft Matter Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, P.O. Box 9504, 2300 RA Leiden, The Netherlands.
Roxanne E KieltykaDepartment of Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.ORCID 0000-0001-9152-1810

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Supramolecular materials provide unique opportunities to mimic both the structure and mechanics of the biopolymer networks that compose the extracellular matrix. However, strategies to modify their filamentous structures in space and time in 3D cell culture to study cell behavior as encountered in development and disease are lacking. We herein disclose a multicomponent squaramide-based supramolecular material whose mechanics and bioactivity can be controlled by light through co-assembly of a 1,2-dithiolane (DT) monomer that forms disulfide cross-links. Remarkably, increases in storage modulus from ∼200 Pa to >10 kPa after stepwise photo-cross-linking can be realized without an initiator while retaining colorlessness and clarity. Moreover, viscoelasticity and plasticity of the supramolecular networks decrease upon photo-irradiation, reducing cellular protrusion formation and motility when performed at the onset of cell culture. When applied during 3D cell culture, force-mediated manipulation is impeded and cells move primarily along earlier formed channels in the materials. Additionally, we show photopatterning of peptide cues in 3D using either a photomask or direct laser writing. We demonstrate that these squaramide-based filamentous materials can be applied to the development of synthetic and biomimetic 3D

Indexed as

Biocompatible MaterialsHydrogelsCell Culture TechniquesCuesExtracellular MatrixBiocompatible MaterialsHydrogels3D cell culturedithiolanehydrogelsphotopatterningsupramolecular

Identifiers

PMID35403421
PMCPMC9026256

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.