ArticleFrontiers in cellular and infection microbiology2022
Microbiome Characterization of Infected Diabetic Foot Ulcers in Association With Clinical Outcomes: Traditional Cultures Versus Molecular Sequencing Methods.
Article in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05770856 (Clinical Metagenomic Next-Generation Sequencing for Microbial Infections in Trauma), which is not on this map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Clinical Metagenomic Next-Generation Sequencing for Microbial Infections in Trauma
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- A systematic review of diabetic foot infections: pathogenesis, diagnosis, and management strategies.Frontiers in clinical diabetes and healthcare · 2024Pooled it
- Antimicrobial Peptides for Diabetic Foot Ulcers and Infections: Current Evidence and Translational Perspectives.International journal of molecular sciences · 2026Review
- Causes of Bacterial Infections and Antibiotic Resistance Profiles in Patients with Chronic Wounds.Microorganisms · 2026Article
- Review
- The microbiological profile of diabetic foot infections: historical trends and relationship with amputation.JAC-antimicrobial resistance · 2026Article
- A Survey of the Microbiome, Culturome and ARG Profile of a Cohort of Chronic Diabetic Foot Lesions.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2026Article
- Metagenomic characterization of infected diabetic foot ulcers in North Africa: microbial diversity, virulome, and resistome profiling.Frontiers in microbiology · 2026Article
- 16S rRNA sequencing and conventional culture provide complementary information in hospitalized patients with chronic lower-limb wounds.Frontiers in cellular and infection microbiology · 2026Article
- Genome-resolved gut metagenomics identifies anFrontiers in immunology · 2026Article
- A longitudinal profiling of microbiome of diabetic foot ulcers shows functional role of microbial communities in wound worsening and chronicity.Current research in microbial sciences · 2026Article
- Anaerobe-associated microbial shifts at infection onset in diabetes-related foot ulcers revealed by longitudinal metagenomics.Frontiers in cellular and infection microbiology · 2026Article
- Periosteal distraction as a new surgical technique for the treatment of senile diabetic foot: A retrospective case analysis.Medicine · 2025Article
- Mechanisms of microbial infection and wound healing in diabetic foot ulcer: pathogenicity in the inflammatory-proliferative phase, chronicity, and treatment strategies.Frontiers in endocrinology · 2025Review
- Management of diabetic foot ulcers: polyherbal formulations and novel delivery systems for herbal active components.Frontiers in pharmacology · 2025Review
- Anaerobes in diabetic foot infections: pathophysiology, epidemiology, virulence, and management.Clinical microbiology reviews · 2024Review
- Clinical perspectives on sampling and processing approaches for the management of infection in diabetic foot ulceration: A qualitative study.International wound journal · 2024Article
- Direct metagenomics investigation of non-surgical hard-to-heal wounds: a review.Annals of clinical microbiology and antimicrobials · 2024Review
- Prokaryotic and eukaryotic skin microbiota modifications triggered by Leishmania infection in localized Cutaneous Leishmaniasis.PLoS neglected tropical diseases · 2024Article
- Microbiome of limb-threatening diabetic foot ulcers indicates the association of fastidious Stenotrophomonas and major amputation.Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi · 2024Article
- Virulence characteristics of Gram-positive bacteria isolated from diabetic foot ulcers.FEMS microbes · 2024Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Infected diabetic foot ulcers (IDFU) are a major complication of diabetes mellitus. These potentially limb-threatening ulcers are challenging to treat due to impaired wound healing characterizing diabetic patients and the complex microbial environment of these ulcers. Aim: To analyze the microbiome of IDFU in association with clinical outcomes. Methods: Wound biopsies from IDFU were obtained from hospitalized patients and were analyzed using traditional microbiology cultures, 16S rRNA sequencing and metagenomic sequencing. Patients' characteristics, culture-based results and sequencing data were analyzed in association with clinical outcomes. Results: A total of 31 patients were enrolled. Gram-negative bacteria dominated the IDFU samples (79%, 59% and 54% of metagenomics, 16S rRNA and cultures results, respectively, p<0.001). 16S rRNA and metagenomic sequencing detected significantly more anaerobic bacteria, as compared to conventional cultures (59% and 76%, respectively vs. 26% in cultures, p=0.001). Culture-based results showed that Conclusion: Sequencing tools uncover the complex biodiversity of IDFU and emphasize the high prevalence of anaerobes and Gram-negative bacteria in these ulcers. Furthermore, sequencing results highlight possible associations among certain genera, species, and bacterial functional genes to clinical outcomes.
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