ArticleFrontiers in microbiology2022
Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis.
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 12 citations in OpenAlex.
- Effects of a low dose of 3-nitrooxypropanol and a combination of direct-fed microbials on methane emission, oral swab bacterial community, and performance in dairy cows.Journal of animal science and biotechnology · 2026Article
- Longitudinal Change in Rumen-Associated Bacterial Communities Following Aso Limonite Supplementation in Japanese Brown Cattle.Animals : an open access journal from MDPI · 2026Article
- Metagenomic Applications to Herbivore Gut Microbiomes: A Comprehensive Review of Microbial Diversity and Host Interactions.Animals : an open access journal from MDPI · 2025Review
- Evaluation of esophageal tubing and buccal swabbing versus rumen cannula to characterize ruminal microbiota in cows fed contrasting diets.Scientific reports · 2025Article
- Gut Microbiota of Ruminants and Monogastric Livestock: An Overview.Animals : an open access journal from MDPI · 2025Review
- State-of-the-art approaches in the investigation of human seminal bacteriome using metagenomic methods.Frontiers in reproductive health · 2025Review
- Glyphosate-microbial interactions: metagenomic insights and future directions.Frontiers in microbiology · 2025Review
- Investigating the establishment of the rumen and oral bacterial communities in beef cattle and assessing the applicability of using the oral bacterial community composition as a proxy for rumen bacterial community structure in cattle.Frontiers in microbiology · 2025Article
- Buccal Swab Samples from Japanese Brown Cattle Fed with Limonite Reveal Altered Rumen Microbiome.Animals : an open access journal from MDPI · 2024Article
- Oral swabs as a proxy for direct ruminal microbiome sampling in Holstein dairy cows is correlated with sample color.Frontiers in microbiology · 2024Article
- The newest Oxford Nanopore R10.4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling.Applied and environmental microbiology · 2023Article
- Ruminant Salivary Microbes: Passenger or Player in the Rumen?Microorganisms · 2023Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Illumina MiSeq platform has been widely used as a standard method for studying the rumen microbiota. However, the low resolution of taxonomic identification is the only disadvantage of MiSeq amplicon sequencing, as it targets a part of the 16S rRNA gene. In the present study, we performed three experiments to establish a high-resolution and high-throughput rumen microbial profiling approach using a combination of MinION platform and buccal swab sample, which is a proxy for rumen contents. In experiment 1, rumen contents and buccal swab samples were collected simultaneously from cannulated cattle (
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