Evidence map›Paper›PMID 35400716›Full record

ArticleMicrobes and environments2022

Degenerate PCR Targeting the Major Capsid Protein Gene of HcRNAV and Related Viruses.

Michiko Takahashi, Kei Wada, Syun-Ichi Urayama, Yuichi Masuda, Keizo Nagasaki

Open access · diamondAbstract read
In one paragraph

Article in Microbes and environments, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Michiko TakahashiFaculty of Science and Technology, Kochi University.
Kei WadaDepartment of Medical Sciences, University of Miyazaki.
Syun-Ichi UrayamaFaculty of Life and Environmental Sciences, University of Tsukuba.
Yuichi MasudaGraduate School of Integrated Arts and Sciences, Kochi University.
Keizo NagasakiFaculty of Science and Technology, Kochi University.
Kōchi University · JPUniversity of Miyazaki · JPUniversity of Tsukuba · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heterocapsa circularisquama RNA virus (HcRNAV) is the only dinoflagellate-infecting RNA virus that has been isolated to date. We herein investigated the diversity of the major capsid protein gene of HcRNAV and related viruses using degenerate PCR and in silico ana-lyses. Diverse sequences related to HcRNAV were successfully amplified from marine sediments. Amplicons contained conserved and variable regions; the latter were predicted to be located on the outer surface of the capsid. Our approach provides insights into the diversity of viruses that are difficult to isolate in the environment and will enhance rapidly growing metagenome sequence repositories.

Indexed as

RNA VirusesVirusesCapsidCapsid ProteinsPolymerase Chain ReactionCapsid Proteinseukaryotic algaemarine virusphytoplanktonstructure modelingviral diversity

Identifiers

PMID35400716
PMCPMC9763038
OpenAlexW4225657697

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.