Evidence map›Paper›PMID 35398454›Full record

ArticleVirus research2022

A novel plant lectin, NTL-125, interferes with SARS-CoV-2 interaction with hACE2.

Anindya Sarkar, Sathi Paul, Charandeep Singh, Nilkanta Chowdhury, Papri Nag, Swarnava Das, Sahil Kumar, Anshul Sharma, Deepjyoti Kumar Das, Dipak Dutta and 6 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Genome-Wide Mining ofCurrent issues in molecular biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Anindya SarkarDivision of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
Sathi PaulDivision of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
Charandeep SinghCSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Nilkanta ChowdhuryDepartment of Biochemistry and Biophysics, University of Kalyani, Kalyani, 741235, India.
Papri NagDivision of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
Swarnava DasDivision of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
Sahil KumarCSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Anshul SharmaCSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Deepjyoti Kumar DasCSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Dipak DuttaCSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Krishan Gopal ThakurCSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Angshuman BagchiDepartment of Biochemistry and Biophysics, University of Kalyani, Kalyani, 741235, India.
Surbhi ShritiDivision of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
Kali P DasFormerly, Department of Chemistry, Bose Institute, 93/1 A.P.C. Road, Kolkata, 700009, India.
Rajesh P RingeCSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Sampa DasDivision of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
Bose Institute · INInstitute of Microbial Technology · INUniversity of Kalyani · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

COVID-19 caused by SARS-CoV-2 virus has had profound impact on the world in the past two years. Intense research is going on to find effective drugs to combat the disease. Over the past year several vaccines were approved for immunization. But SARS-CoV-2 being an RNA virus is continuously mutating to generate new variants, some of which develop features of immune escape. This raised serious doubts over the long-term efficacy of the vaccines. We have identified a unique mannose binding plant lectin from Narcissus tazetta bulb, NTL-125, which effectively inhibits SARS-CoV-2 replication in Vero-E6 cell line. In silico docking studies revealed that NTL-125 has strong affinity to viral Spike RBD protein, preventing it from attaching to hACE2 receptor, the gateway to cellular entry. Binding analyses revealed that all the mutant variants of Spike protein also have stronger affinity for NTL-125 than hACE2. The unique α-helical tail of NTL-125 plays most important role in binding to RBD of Spike. NTL-125 also interacts effectively with some glycan moieties of S-protein in addition to amino acid residues adding to the binding strength. Thus, NTL-125 is a highly potential antiviral compound of natural origin against SARS-CoV-2 and may serve as an important therapeutic for management of COVID-19.

Indexed as

Angiotensin-Converting Enzyme 2Plant LectinsSARS-CoV-2Spike Glycoprotein, CoronavirusCOVID-19HumansNarcissusProtein BindingACE2 protein, humanAngiotensin-Converting Enzyme 2Plant LectinsSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Molecular dockingMutant variants, Narcissus tazetta lectinSARS-CoV-2Spike Protein

Identifiers

PMID35398454
PMCPMC8988448
OpenAlexW4223478803

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.