Evidence map›Paper›PMID 35398258›Full record

ArticleFungal genetics and biology : FG & B2022

Characterization of a silent azaphilone biosynthesis gene cluster in Aspergillus terreus NIH 2624.

Wei-Wen Sun, Chi-Ying Li, Yi-Ming Chiang, Tzu-Shyang Lin, Shauna Warren, Fang-Rong Chang, Clay C C Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fungal genetics and biology : FG & B, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Potential of Secondary Metabolites ofJournal of fungi (Basel, Switzerland) · 2023
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Wei-Wen SunDepartment of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy, 1985 Zonal Avenue, Los Angeles, CA 90089, USA.
Chi-Ying LiDepartment of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy, 1985 Zonal Avenue, Los Angeles, CA 90089, USA.
Yi-Ming ChiangDepartment of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy, 1985 Zonal Avenue, Los Angeles, CA 90089, USA; Graduate Institute of Pharmaceutical Science, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan.
Tzu-Shyang LinDepartment of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy, 1985 Zonal Avenue, Los Angeles, CA 90089, USA.
Shauna WarrenDepartment of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy, 1985 Zonal Avenue, Los Angeles, CA 90089, USA.
Fang-Rong ChangGraduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: aaronfrc@kmu.edu.tw.
Clay C C WangDepartment of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy, 1985 Zonal Avenue, Los Angeles, CA 90089, USA; Department of Chemistry, University of Southern California, College of Letters, Arts, and Sciences, Los Angeles, CA 90089, USA. Electronic address: clayw@usc.edu.
University of Southern California · USKaohsiung Medical University · TW

Funding

Exploiting interspecies/interkingdom communication for the discovery and development of novel natural products antifungal therapeuticsR21AI127640 · NIAID · UNIVERSITY OF SOUTHERN CALIFORNIA · PI WANG, CLAY CC · 2017 to 2018
$449k
NIAID NIH HHS R21 AI127640
6 · The paper itself

Abstract

Filamentous fungal secondary metabolites are an important source of bioactive components. Genome sequencing ofAspergillus terreusrevealed many silent secondary metabolite biosynthetic gene clusters presumed to be involved in producing secondary metabolites. Activation of silent gene clusters through overexpressing a pathway-specific regulator is an effective avenue for discovering novel fungal secondary metabolites. Replacement of the native promoter of the pathway-specific activator with the inducible Tet-on system to activate thetazpathway led to the discovery of a series of azaphilone secondary metabolites, among which azaterrilone A (1) was purified and identified for the first time. Genetic deletion of core PKS genes and transcriptional analysis further characterized thetazgene cluster to consist of 16 genes with the NR-PKS and the HR-PKS collaborating in a convergent mode. Based on the putative gene functions and the characterized compounds structural information, a biosynthetic pathway of azaterrilone A (1) was proposed.

Indexed as

AspergillusMultigene FamilyBenzopyransPigments, BiologicalPolyketide SynthasesazaphiloneBenzopyransPigments, BiologicalPolyketide SynthasesAspergillus terreusAzaphiloneBiosynthesisInducible promoterPolyketide synthaseSecondary metabolitesTet-on system

Identifiers

PMID35398258
PMCPMC9701353
OpenAlexW4223446675

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.