Evidence map›Paper›PMID 35397717›Full record

ArticleWorld journal of microbiology & biotechnology2022

Heterologous expression and characterization of two novel glucanases derived from sheep rumen microbiota.

De-Ying Gao, Xiao-Bao Sun, Ying Fang, Bo He, Jun-Hong Wang, Jian-Xin Liu, Jia-Kun Wang, Qian Wang

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of microbiology & biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Microbial β-glucanases: production, properties, and engineering.World journal of microbiology & biotechnology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

De-Ying GaoKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, 310058, China.
Xiao-Bao SunKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, 310058, China.
Ying FangAnimal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen, 518000, China.
Bo HeKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, 310058, China.
Jun-Hong WangKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, 310058, China.
Jian-Xin LiuKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, 310058, China.
Jia-Kun WangKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, 310058, China. jiakunwang@zju.edu.cn.
Qian WangKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, 310058, China. Emirate14@zju.edu.cn.ORCID http://orcid.org/0000-0001-6804-4083
Ministry of Education · NZZhejiang University · CNShanghai Jiao Tong University · CNShenzhen Academy of Inspection and Quarantine · CN

Funding

National Natural Science Foundation of China 31902201
6 · The paper itself

Abstract

β-Glucanases are a suite of glycoside hydrolases that depolymerize β-glucan into cellooligosaccharides and/or monosaccharides and have been widely used as feed additives in livestock. In this study, two novel glucanase genes, IDSGluc5-26 and IDSGluc5-37, derived from sheep rumen microbiota, were expressed and functionally characterized. The optimal temperatures/pH of recombinant IDSGLUC5-26 and IDSGLUC5-37 were 50 °C/5.0 and 40 °C/6.0, respectively. Notably, IDSGLUC5-26 showed considerable stability under acidic conditions. Both IDSGLUC5-26 and IDSGLUC5-37 showed the highest activities toward barley β-glucan, with V

Indexed as

beta-GlucansMicrobiotaAmino Acid SequenceAnimalsGlycoside HydrolasesRecombinant ProteinsRumenSheepSubstrate Specificitybeta-GlucansGlycoside HydrolasesRecombinant ProteinsAcid-stableGlucanaseHeterologous expressionRumen microbeSubstrate hydrolysis

Identifiers

PMID35397717
OpenAlexW4226072634

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.