Evidence map›Paper›PMID 35397625›Full record

ArticleScientific reports2022

Phenanthrene-enriched extract from Eulophia macrobulbon using subcritical dimethyl ether for phosphodiesterase-5A1 inhibition.

Jukkarin Srivilai, Panatpong Boonnoun, Tongchai Saesong, Chitaporn Pingyod, Nattiya Chaichamnong, Jinutda Engsuwan, Prapapan Temkitthawon, C Norman Scholfield, Nitra Nuengchamnong, Nantaka Khorana and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
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Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Jukkarin SrivilaiResearch and Innovation Center in Cosmetic Sciences and Natural Products, Department of Cosmetic Sciences, School of Pharmaceutical Sciences, University of Phayao, Phayao, 56000, Thailand.
Panatpong BoonnounChemical Engineering Program, Department of Industrial Engineering, Faculty of Engineering, Naresuan University, Phitsanulok, 65000, Thailand.
Tongchai SaesongDepartment of Pharmaceutical Chemistry and Pharmacognosy, Center of Excellence in Cannabis Research, Faculty of Pharmaceutical Sciences, Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, 65000, Thailand.
Chitaporn PingyodDepartment of Pharmaceutical Chemistry and Pharmacognosy, Center of Excellence in Cannabis Research, Faculty of Pharmaceutical Sciences, Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, 65000, Thailand.
Nattiya ChaichamnongDivision of Applied Thai Traditional Medicine, Faculty of Public Health, Naresuan University, Phitsanulok, 65000, Thailand.
Jinutda EngsuwanResearch and Innovation Center in Cosmetic Sciences and Natural Products, Department of Cosmetic Sciences, School of Pharmaceutical Sciences, University of Phayao, Phayao, 56000, Thailand.
Prapapan TemkitthawonDepartment of Pharmaceutical Chemistry and Pharmacognosy, Center of Excellence in Cannabis Research, Faculty of Pharmaceutical Sciences, Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, 65000, Thailand.
C Norman ScholfieldAkkhraratchakumari Veterinary College, Walailak University, Thasala District, Nakhon Si Thammarat, 80160, Thailand.
Nitra NuengchamnongScience Laboratory Centre, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand.
Nantaka KhoranaResearch and Innovation Center in Cosmetic Sciences and Natural Products, Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, University of Phayao, Phayao, 56000, Thailand.
Kornkanok IngkaninanDepartment of Pharmaceutical Chemistry and Pharmacognosy, Center of Excellence in Cannabis Research, Faculty of Pharmaceutical Sciences, Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, 65000, Thailand. k_ingkaninan@yahoo.com.
Naresuan University · THUniversity of Phayao · THWalailak University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Eulophia macrobulbon (E.C.Parish & Rchb.f.) Hook.f. contains a natural PDE5A1 inhibitor, phenanthrene, 1-(4'-hydroxybenzyl)-4,8- dimethoxyphenanthrene-2,7-diol (HDP), a potential agent for the treatment of erectile dysfunction. The aim of this study was to improve the extraction efficiency of HDP from E. macrobulbon by using a more environmentally friendly extraction method, subcritical liquid dimethyl ether extraction (sDME), instead of classical solvent extraction (CSE) and ultrasound-assisted extraction (UAE). The efficiency and quality of the extracts obtained were evaluated using the following criteria: %process yield; solvent amount; extraction time; temperature; %HDP content by LC-MS, bioactivity as inhibition of phosphodiesterase-5A1 (PDE5A1) by radio-enzymatic assay; and chemical profiles by LC-QTOF-MS. sDME provided the highest content of HDP in the extract at 4.47%, much higher than the use of ethanol (0.4-0.5%), ethyl acetate (1.2-1.7%), or dichloromethane (0.7-1.4%). The process yield for sDME (1.5-2.7%) was similar to or lower than the other solvents (0.9-17%), but as long as the process yield is not prohibitively low, the concentration is a more important measure for clinical use. The optimal conditions for sDME extraction were: Extraction time, 40 min; 200% water as co-solvent; sample-to-solvent ratio of 1:8; temperature, 35 °C. Phenanthrene aglycone and glycoside derivatives were the major constituents of the sDME extracts and lesser amounts of phenolic compounds and sugars. The inhibition of PDE5A1 by sDME (IC

Indexed as

OrchidaceaePhosphoric Diester HydrolasesEthanolMethyl EthersPhenanthrenesPlant ExtractsSolventsdimethyl etherEthanolMethyl EthersphenanthrenePhenanthrenesPhosphoric Diester HydrolasesPlant ExtractsSolvents

Identifiers

PMID35397625
PMCPMC8994770
OpenAlexW4226190320

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.