Evidence map›Paper›PMID 35397519›Full record

ArticleBMC musculoskeletal disorders2022

MicroRNA-455-3p regulates proliferation and osteoclast differentiation of RAW264.7 cells by targeting PTEN.

Xiaolu Zhang, Liangming Wang, Nianlai Huang, Yiqiang Zheng, Liquan Cai, Qingfeng Ke, Shiqiang Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC musculoskeletal disorders, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Xiaolu Zhang *The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China.
Liangming Wang *The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China.
Nianlai HuangThe Second Clinical Medical College, Fujian Medical University, Fuzhou, 350000, China.
Yiqiang ZhengThe Second Clinical Medical College, Fujian Medical University, Fuzhou, 350000, China.
Liquan CaiThe Second Clinical Medical College, Fujian Medical University, Fuzhou, 350000, China.
Qingfeng KeThe Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China.
Shiqiang WuThe Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China. wushiqiang@fjmu.edu.cn.
Fujian Medical University · CNSecond Affiliated Hospital of Fujian Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMacrophages are one of the important cells in immune system. In this article, we aim to explore the regulatory role of miR-455-3p on proliferation and osteoblast differentiation of RAW264.7 cells.

methodsExpression levels of genes and proteins in cells were tested via qRT-PCR and western blot. The targeted correlation between miR-455-3p and PTEN was identified by luciferase analysis. MTT assay and flow cytometry were applied to detect the proliferation and apoptosis of cells. Osteoclastogenesis was completed by stimulating RAW 264.7 cells with RANKL. Tartrate-resistant acid phosphatase (TRAP) activity in different groups of cells were assessed.

resultsFirstly, we determined that up-regulation of miR-455-3p promoted the proliferation and inhibited apoptosis of RAW 264.7 cells. MiR-455-3p deficiency played opposite effect in RAW 264.7 cells. Additionally, osteoclastogenesis-related factors (TRAP, CTSK and NFATc1) expression levels were remarkably up-regulated in miR-455-3p-mimic group of RAW264.7 cells treated with RANKL, but decreased in inhibitor group. Luciferase assay proved that miR-455-3p targeted PTEN. We took a further step and found overexpression of PTEN significantly inhibited the increased proliferation and osteoblast differentiation of RAW264.7 cells induced by miR-455-3p.

conclusionsOur findings supported basic to explore the molecular mechanism of proliferation and osteoblast differentiation of RAW264.7 cells.

Indexed as

MicroRNAsOsteoclastsOsteogenesisPTEN PhosphohydrolaseAnimalsCell ProliferationMiceRAW 264.7 CellsMicroRNAsMIRN455 microRNA, mousePTEN PhosphohydrolasePTEN protein, humanCell proliferationmiR-455-3pOsteoclast differentiationPTENRAW264.7

Identifiers

PMID35397519
PMCPMC8994399
OpenAlexW4223492190

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.