Evidence map›Paper›PMID 35397087›Full record

ArticleMolecular biology reports2022

miR-211-3p enhances induction chemotherapy insensitivity by upregulating CSF2/CCL20/TNF signaling in hypopharyngeal squamous cell carcinoma.

Lingwa Wang, Ru Wang, Tianqiao Huang, Yifan Yang, Ling Feng, Jugao Fang

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Lingwa Wang *Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
Ru Wang *Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
Tianqiao HuangDepartment of Otolaryngology Head and Neck Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.
Yifan YangDepartment of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
Ling FengDepartment of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. floscar@126.com.
Jugao FangDepartment of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. fangjugao@163.com.ORCID http://orcid.org/0000-0002-0188-7120
Beijing Tongren Hospital · CNQingdao University · CN

Funding

Beijing Hospitals Authority Youth Programme QML20200205Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support DFL20180202Beijing Municipal Administration of Hospitals Incubating Program PX2021008Beijing Natural Science Foundation Program and Scientific Research Key Program of Beijing Municipal Commission of Education KZ201910025034National Key R&D Program of China 2020YFB1312805National Natural Science Foundation of China 82002880
6 · The paper itself

Abstract

purposeTo investigate the potential mechanisms of miR-211-3p on induction chemotherapy (IC) sensitivity in hypopharyngeal squamous cell carcinoma (HSCC).

methodsqRT-PCR was assessed to compare the miR-211-3p expression between IC sensitive and insensitive tumor tissues. The MTT assay was performed to analyze cell proliferation and viability to paclitaxel after alteration of miR-211-3p. Flow cytometry assay was conducted to explore cell apoptosis. Transwell assay was used to explore the effect of miR-211-3p on cell migration. Transcriptome sequencing was then performed to select differentially expressed genes (DEGs) after over-expression of miR-211-3p. GO and KEGG enrichment analyses were conducted to annotate DEGs. PPI analysis was conducted to screen candidate genes. The differential expression and survival status of candidate genes were further validated in TCGA-HNSCC data. The single sample GSEA method was used to investigate the association between downstream genes and immune cell infiltration.

resultsmiR-211-3p was up-regulated in IC insensitive larynx-hypopharyngeal tumor tissues. Over-expression of miR-211-3p promoted cell proliferation and migration, and inhibited apoptosis. The IC

conclusionThe miR-211-3p could promote HSCC progression and upregulate CSF2/CCL20/TNF signaling to promote IC insensitivity in HSCC, which may provide new ideas for HSCC therapy.

Indexed as

Carcinoma, Squamous CellHead and Neck NeoplasmsMicroRNAsCell Line, TumorCell MovementCell ProliferationChemokine CCL20Gene Expression Regulation, NeoplasticGranulocyte-Macrophage Colony-Stimulating FactorHumansInduction ChemotherapyLigandsPaclitaxelSquamous Cell Carcinoma of Head and NeckTumor Necrosis FactorsCCL20 protein, humanChemokine CCL20CSF2 protein, humanGranulocyte-Macrophage Colony-Stimulating FactorLigandsMicroRNAsMIRN211 microRNA, humanPaclitaxelTumor Necrosis FactorsHypopharyngeal squamous cell carcinoma (HSCC)Induction chemotherapy (IC)miR-211-3p

Identifiers

PMID35397087
OpenAlexW4225498466

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.