Evidence map›Paper›PMID 35397012›Full record

ArticleArchives of microbiology2022

Klebsiella oxytoca: an efficient pyrene-degrading bacterial strain isolated from petroleum-contaminated soil.

Abdulkhaleg M Alfaify, Mushtaq Ahmad Mir, Sulaiman A Alrumman

Abstract read
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In one paragraph

Article in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Abdulkhaleg M AlfaifyDepartment of Biology, College of Science, King Khalid University, Abha, Saudi Arabia.
Mushtaq Ahmad MirDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, P. O. Box 3665, Abha, 61421, Saudi Arabia. mmir@kku.edu.sa.ORCID http://orcid.org/0000-0002-4388-1165
Sulaiman A AlrummanDepartment of Biology, College of Science, King Khalid University, Abha, Saudi Arabia.
King Khalid University · SA

Funding

King Abdul-Aziz City for Science and Technology (KACST) 1-17-01-010-0012
6 · The paper itself

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are the hazardous xenobiotic agents of oil production. One of the methods to eliminate hazardous compounds is bioremediation, which is the most efficient and cost-effective method to eliminate the harmful byproducts of crude petroleum processing. In this study, five pure bacterial isolates were isolated from petroleum-contaminated soil, four of which showed a robust growth on the PAH pyrene, as a sole carbon source. Various methods viz mass spectroscopy, biochemical assays, and 16S RNA sequencing employed to identify the isolates ascertained the consistent identification of Klebsiella oxytoca by all three methods. Scanning electron microscopy and Gram staining further demonstrated the characterization of the K. oxytoca. High-performance liquid chromatography of the culture supernatant of K. oxytoca grown in pyrene containing media showed that the cells started utilizing pyrene from the 6th day onwards and by the 12th day of growth, 70% of the pyrene was completely degraded. A genome search for the genes predicted to be involved in pyrene degradation using Kyoto Encyclopedia of Genes and Genomes (KEGG) confirmed their presence in the genome of K. oxytoca. These results suggest that K. oxytoca would be a suitable candidate for removing soil aromatic hydrocarbons.

Indexed as

PetroleumPolycyclic Aromatic HydrocarbonsSoil PollutantsBacteriaBiodegradation, EnvironmentalKlebsiella oxytocaPyrenesRNA, Ribosomal, 16SSoilSoil MicrobiologyPetroleumPolycyclic Aromatic HydrocarbonspyrenePyrenesRNA, Ribosomal, 16SSoilSoil PollutantsBiodegradationContaminated soilPetroleumPolycyclic aromatic hydrocarbonsPyrene

Identifiers

PMID35397012
OpenAlexW4223516719

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.