ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022
A Long-Term Clearing Cranial Window for Longitudinal Imaging of Cortical and Calvarial Ischemic Injury through the Intact Skull.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Mapping Brain Spatial Metabolic Atlas Reveals Anesthesia-Induced Region-Specific Alterations.Neuroscience bulletin · 2026Article
- Choosing a skull clearing technique for chronic mesoscopic optical imaging in awake mice.Neurophotonics · 2026Article
- Noninvasive photoacoustic computed mesoscopy for longitudinal brain imaging.Science advances · 2026Article
- Neuro-lymphaphotonics opens new horizons of the future technologies for the therapy of brain diseases.Theranostics · 2026Review
- Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice.Nature communications · 2025Article
- An optical clearing imaging window: Realization of mouse brain imaging and manipulation through scalp and skull.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- On-chip clearing for live imaging of 3D cell cultures.Biomedical optics express · 2023Article
- A Long-Term Clearing Cranial Window for Longitudinal Imaging of Cortical and Calvarial Ischemic Injury through the Intact Skull.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Optical clearing imaging assisted evaluation of urokinase thrombolytic therapy on cerebral vessels with different sizes.Biomedical optics express · 2022Article
- Systematic analysis of brain and skull ischemic injury expression profiles reveals associations of the tumor immune microenvironment and cell death with ischemic stroke.Frontiers in immunology · 2022Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Skull is a reservoir for supplying immune cells that mediate brain immune surveillance. However, during intravital optical imaging of brain, conventional cranial windows requiring skull thinning or removal disrupt brain immunity integrity. Here, a novel long-term clearing cranial window (LCCW) based on the intact skull, dedicated to chronic skull transparency maintenance, is proposed. It significantly improves optical imaging resolution and depth, by which the cortical and calvarial vascular injury and regeneration processes after ischemic injury are longitudinally monitored in awake mice. Results show that calvarial blood vessels recover earlier than the cortex. And the transcriptome analysis reveals that gene expression patterns and immune cells abundances exist substantial differences between brain and skull after ischemic injury, which may be one of the causes for the time lag between their vascular recovery. These findings bring great enlightenment to vascular regeneration and reconstruction. Moreover, LCCW provides a minimally invasive approach for imaging the brain and skull bone marrow.
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Registered trials
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