ArticleScientific reports2022
A Saccharomyces eubayanus haploid resource for research studies.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Article
- Adaptive laboratory evolution of maltose utilization in wild Irish isolates of Saccharomyces eubayanus.FEMS yeast research · 2026Article
- Contrasting mutation patterns in haploid and diploid cells from two yeast species.bioRxiv : the preprint server for biology · 2025Article
- Experimental evolution and hybridization enhance the fermentative capacity of wild Saccharomyces eubayanus strains.FEMS yeast research · 2025Article
- Isolation and characterization of haploid heterothallic beer yeasts.Applied microbiology and biotechnology · 2025Article
- Horizontal Transfer and Recombination Fuel Ty4 Retrotransposon Evolution in Saccharomyces.Genome biology and evolution · 2025Article
- An integrative taxonomy approach reveals Saccharomyces chiloensis sp. nov. as a newly discovered species from Coastal Patagonia.PLoS genetics · 2024Article
- Wild Patagonian yeast improve the evolutionary potential of novel interspecific hybrid strains for lager brewing.PLoS genetics · 2024Article
- Ploidy evolution in a wild yeast is linked to an interaction between cell type and metabolism.PLoS biology · 2023Article
- Natural Variation in Diauxic Shift between PatagonianmSystems · 2022Article
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Authors and funding
10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Since its identification, Saccharomyces eubayanus has been recognized as the missing parent of the lager hybrid, S. pastorianus. This wild yeast has never been isolated from fermentation environments, thus representing an interesting candidate for evolutionary, ecological and genetic studies. However, it is imperative to develop additional molecular genetics tools to ease manipulation and thus facilitate future studies. With this in mind, we generated a collection of stable haploid strains representative of three main lineages described in S. eubayanus (PB-1, PB-2 and PB-3), by deleting the HO gene using CRISPR-Cas9 and tetrad micromanipulation. Phenotypic characterization under different conditions demonstrated that the haploid derivates were extremely similar to their parental strains. Genomic analysis in three strains highlighted a likely low frequency of off-targets, and sequencing of a single tetrad evidenced no structural variants in any of the haploid spores. Finally, we demonstrate the utilization of the haploid set by challenging the strains under mass-mating conditions. In this way, we found that S. eubayanus under liquid conditions has a preference to remain in a haploid state, unlike S. cerevisiae that mates rapidly. This haploid resource is a novel set of strains for future yeast molecular genetics studies.
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