Evidence map›Paper›PMID 35395782›Full record

ArticleStem cell research & therapy2022

Exosomes from adipose-derived stem cells regulate M1/M2 macrophage phenotypic polarization to promote bone healing via miR-451a/MIF.

Rui Li, Dize Li, Huanan Wang, Kaiwen Chen, Si Wang, Jie Xu, Ping Ji

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
119citing papers in PubMed, 2 pooled it
14.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

119 citing papers in PubMed, 2 syntheses or guidelines pooled it, 179 citations in OpenAlex.

  1. Pooled it
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  3. Extracellular Vesicles and Their Role in Osteogenesis.Bioengineering (Basel, Switzerland) · 2026
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59 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Rui Li *Department of Pediatric Dentistry, The College of Stomatology, Chongqing Medical University, No. 426, North Songshi Road, Yubei District, Chongqing, 401147, People's Republic of China.
Dize Li *Department of Pediatric Dentistry, The College of Stomatology, Chongqing Medical University, No. 426, North Songshi Road, Yubei District, Chongqing, 401147, People's Republic of China.
Huanan WangKey State Laboratory of Fine Chemicals, School of Bioengineering, Dalian University of Technology, Dalian, 116023, People's Republic of China.
Kaiwen ChenKey State Laboratory of Fine Chemicals, School of Bioengineering, Dalian University of Technology, Dalian, 116023, People's Republic of China.
Si WangDepartment of Pediatric Dentistry, The College of Stomatology, Chongqing Medical University, No. 426, North Songshi Road, Yubei District, Chongqing, 401147, People's Republic of China. 501112@cqmu.edu.cn.
Jie XuDepartment of Pediatric Dentistry, The College of Stomatology, Chongqing Medical University, No. 426, North Songshi Road, Yubei District, Chongqing, 401147, People's Republic of China. xujie@hospital.cqmu.edu.cn.ORCID 0000-0003-2435-0382
Ping JiDepartment of Pediatric Dentistry, The College of Stomatology, Chongqing Medical University, No. 426, North Songshi Road, Yubei District, Chongqing, 401147, People's Republic of China. jiping@hospital.cqmu.edu.cn.
Dalian University of Technology · CNStomatological Hospital of Chongqing Medical University · CNThe Affiliated Yongchuan Hospital of Chongqing Medical University · CNChongqing University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesBone defects caused by diseases and trauma are usually accompanied by inflammation, and the implantation of biomaterials as a common repair method has also been found to cause inflammatory reactions, which affect bone metabolism and new bone formation. This study investigated whether exosomes from adipose-derived stem cells (ADSC-Exos) plays an immunomodulatory role in traumatic bone defects and elucidated the underlying mechanisms.

methodsADSC-Exos were loaded by a biomaterial named gelatine nanoparticles (GNPs), physical and chemical properties were analysed by zeta potential, surface topography and rheology. A rat model of skull defect was used for our in vivo studies, and micro-CT and histological staining were used to analyse histological changes in the bone defect area. RT-qPCR and western blotting were performed to verify that ADSC-Exos could regulate M1/M2 macrophage polarization. MicroRNA (miRNA) array analysis was conducted to determine the miRNA expression profiles of ADSC-Exos. After macrophages were treated with a miR-451a mimic, miR-451a inhibitor and ISO-1, the relative expression of genes and proteins was measured by RT-qPCR and western blotting.

resultsIn vivo, micro-CT and histological staining showed that exosome-loaded GNPs (GNP-Exos) hydrogel, with good biocompatibility and strong mechanical adaptability, exhibited immunomodulatory effect mainly by regulating macrophage immunity and promoting bone tissue healing. Immunofluorescence further indicated that ADSC-Exos reduced M1 marker (iNOS) expression and increased M2 marker (CD206) expression. Moreover, in vitro studies, western blotting and RT-qPCR showed that ADSC-Exos inhibited M1 macrophage marker expression and upregulated M2 macrophage marker expression. MiR-451a was enriched in ADSC-Exos and targeted macrophage migration inhibitory factor (MIF). Macrophages treated with the miR-451a mimic showed lower expression of M1 markers. In contrast, miR-451a inhibitor treatment upregulated the expression of M1 markers and downregulated the expression of M2 markers, while ISO-1 (a MIF inhibitor) treatment upregulated miR-451a expression and downregulated M1 macrophage marker expression.

conclusionGNP-Exos can effectively regulate bone immune metabolism and further promote bone healing partly through immune regulation of miR-451a, which may provide a therapeutic direction for bone repair.

Indexed as

Bone and BonesExosomesMacrophage Migration-Inhibitory FactorsMacrophagesMicroRNAsAnimalsInflammationIntramolecular OxidoreductasesRatsStem CellsIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMicroRNAsMif protein, ratMIRN451A microRNA, ratAdipose-derived stem cellsBone healingExosomesMacrophagesMiRNA

Identifiers

PMID35395782
PMCPMC8994256
OpenAlexW4225753840

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.