Evidence map›Paper›PMID 35395730›Full record

ArticleBMC microbiology2022

Effects of reduced gag cleavage efficiency on HIV-1 Gag-Pol package.

Yi-Ru Lin, Shih-Ming Chu, Fu-Hsien Yu, Kuo-Jung Huang, Chin-Tien Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yi-Ru LinInstitute of Clinical Medicine, National Yang Ming Chiao Tung University School of Medicine, 112, Taipei, Taiwan.
Shih-Ming ChuInstitute of Clinical Medicine, National Yang Ming Chiao Tung University School of Medicine, 112, Taipei, Taiwan.
Fu-Hsien YuInstitute of Clinical Medicine, National Yang Ming Chiao Tung University School of Medicine, 112, Taipei, Taiwan.
Kuo-Jung HuangDivision of Clinical Research, Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
Chin-Tien WangInstitute of Clinical Medicine, National Yang Ming Chiao Tung University School of Medicine, 112, Taipei, Taiwan. chintien@nycu.edu.tw.ORCID 0000-0003-2231-4492
National Yang Ming Chiao Tung University · TWTaipei Veterans General Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHIV-1 pol, which encodes enzymes required for virus replication, is initially translated as a Gag-Pol fusion protein. Gag-Pol is incorporated into virions via interactions with Gag precursor Pr55

resultsWe engineered PR cleavage-blocking Gag mutations with the potential to significantly reduce Gag processing efficiency. Such mutations may mitigate the negative effects of enhanced PR activation on virus assembly and Gag-Pol packaging due to an RT dimerization enhancer or leucine zipper dimerization motif. When co-expressed with Pr55

conclusionsOur data suggest that the amount of HIV-1 Gag-Pol or Pol viral incorporation is largely dependent on virus particle production, and that cleavage blocking in the Gag-Pol N-terminal Gag domain does not exert significant impacts on Pol packaging.

Indexed as

HIV-1Fusion Proteins, gag-polLeucine ZippersVirionVirus AssemblyFusion Proteins, gag-polGagGag cleavageGag-PolHIV-1ProteaseVirus assembly

Identifiers

PMID35395730
PMCPMC8994222
OpenAlexW4225578357

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.