Evidence map›Paper›PMID 35395188›Full record

ArticleCell stem cell2022

Human pluripotent stem cell-derived myogenic progenitors undergo maturation to quiescent satellite cells upon engraftment.

Congshan Sun, Suraj Kannan, In Young Choi, HoTae Lim, Hao Zhang, Grace S Chen, Nancy Zhang, Seong-Hyun Park, Carlo Serra, Shama R Iyer and 7 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stem cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
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  15. Review
  16. Article
  17. Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles.International journal of molecular sciences · 2023
    Review
  18. The emergence of the stem cell niche.Trends in cell biology · 2023
    Review
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 2 countries.

Congshan SunDepartments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Center for Genetic Muscle Disorders, The Kennedy Krieger Institute, Baltimore, MD 21205, USA.
Suraj KannanInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Biomedical Engineering and Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
In Young ChoiDepartments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
HoTae LimDepartments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Hao ZhangDepartment of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
Grace S ChenKrieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD 21218, USA.
Nancy ZhangKrieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD 21218, USA.
Seong-Hyun ParkDepartments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Carlo SerraDepartments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Center for Genetic Muscle Disorders, The Kennedy Krieger Institute, Baltimore, MD 21205, USA.
Shama R IyerDepartment of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Thomas E LloydDepartments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Chulan KwonInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Biomedical Engineering and Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Richard M LoveringDepartment of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Su Bin LimDepartment of Biochemistry and Molecular Biology, Ajou University School of Medicine, Suwon, 16499, South Korea.
Peter AndersenDivision of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Kathryn R WagnerDepartments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Center for Genetic Muscle Disorders, The Kennedy Krieger Institute, Baltimore, MD 21205, USA. Electronic address: wagnerk@kennedykrieger.org.
Gabsang LeeDepartments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: glee48@jhmi.edu.
Johns Hopkins University · USKennedy Krieger Institute · USUniversity of Maryland, Baltimore · USAjou University · KR

Funding

Medical Scientist Training ProgramT32GM136577 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI ANDREA L COX · 2020 to 2026
$13.4M
Engineering Clinical Trials on a Chip for Dystrophin-Deficient Muscular DystrophyUH3TR003271 · NCATS · JOHNS HOPKINS UNIVERSITY · PI KASS, DAVID ALAN, KIM, DEOK-HO · 2022 to 2024
$2.4M
Transcriptomic Entropy to Quantify Maturation of PSC-Derived CardiomyocytesR01HL156947 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI KIM, DEOK-HO, KWON, CHULAN · 2021 to 2024
$2.3M
Pathogenesis and treatment of sporadic Inclusion Body Myositis in mouse models.R01AR076390 · NIAMS · JOHNS HOPKINS UNIVERSITY · PI LLOYD, THOMAS E., WONG, PHILIP C · 2020 to 2024
$2.2M
Interrogating functional and molecular properties of PAX7+ putative skeletal muscle stem/progenitor cells derived from human iPSCs of healthy donors and Duchenne muscular dystrophy patientsR01AR070751 · NIAMS · JOHNS HOPKINS UNIVERSITY · PI LEE, GABSANG · 2017 to 2021
$1.8M
Cell extrinsic factors' roles on direct conversion to human induced neural crestR01NS093213 · NINDS · JOHNS HOPKINS UNIVERSITY · PI LEE, GABSANG · 2015 to 2019
$1.8M
Engineering Clinical Trials on a Chip for Dystrophin-Deficient Muscular DystrophyUG3TR003271 · NCATS · JOHNS HOPKINS UNIVERSITY · PI KASS, DAVID ALAN, KIM, DEOK-HO · 2020 to 2021
$1.6M
Altered nucleus-cytoskeleton coupling in dystrophic muscleK01AR074048 · NIAMS · UNIVERSITY OF MARYLAND BALTIMORE · PI IYER, SHAMA RAJAN · 2019 to 2023
$420k
NCATS NIH HHS UG3 TR003271NCATS NIH HHS UH3 TR003271NHLBI NIH HHS R01 HL156947NIAMS NIH HHS K01 AR074048NIAMS NIH HHS R01 AR070751NIAMS NIH HHS R01 AR076390NIGMS NIH HHS T32 GM136577NINDS NIH HHS R01 NS093213
6 · The paper itself

Abstract

Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation is a promising therapeutic approach for a variety of degenerative muscle disorders. Here, using an MPC-specific fluorescent reporter system (PAX7::GFP), we demonstrate that hPSC-derived MPCs can contribute to the regeneration of myofibers in mice following local injury and in mice deficient of dystrophin (mdx). We also demonstrate that a subset of PAX7::GFP MPCs engraft within the basal lamina of regenerated myofibers, adopt a quiescent state, and contribute to regeneration upon reinjury and in mdx mouse models. This subset of PAX7::GFP MPCs undergo a maturation process and remodel their molecular characteristics to resemble those of late-stage fetal MPCs/adult satellite cells following in vivo engraftment. These in-vivo-matured PAX7::GFP MPCs retain a cell-autonomous ability to regenerate and can repopulate in the niche of secondary recipient mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.

Indexed as

Pluripotent Stem CellsSatellite Cells, Skeletal MuscleAnimalsCell DifferentiationDystrophinHumansMiceMice, Inbred mdxMuscle DevelopmentMuscle, SkeletalMyoblastsStem Cell TransplantationDystrophinDuchenne muscular dystrophymdx mousepluripotent stem cellsquiescent stem cellsskeletal muscle stem cells

Identifiers

PMID35395188
PMCPMC9000524
OpenAlexW4223454823

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.