Evidence map›Paper›PMID 35394600›Full record

ArticleMolecular biology reports2022

Genetic diversity and relationships among Nopalea sp. and Opuntia spp. accessions revealed by RAPD, ISSR and ITS molecular markers.

Janieire Dorlamis Cordeiro Bezerra, Alberício Pereira de Andrade, Mailson Monteiro do Rêgo, Divan Soares da Silva, José Ribamar Silva do Nascimento Júnior, Fernando Dos Santos Araújo, Roberta de Lima Valença, Elizanilda Ramalho do Rêgo, Angela Maria Dos Santos Pessoa, Riselane de Lucena Alcântara Bruno and 1 more

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 9 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Janieire Dorlamis Cordeiro BezerraDepartamento de Zootecnia, Universidade Federal da Paraíba, Areia, Paraíba, Brazil.ORCID http://orcid.org/0000-0002-2793-1273
Alberício Pereira de AndradeUniversidade Federal do Agreste de Pernambuco, Av. Bom Pastor s/n - Boa Vista, Garanhuns, Pernambuco, 55292-270, Brazil.ORCID http://orcid.org/0000-0002-1223-394X
Mailson Monteiro do RêgoDepartamento de Fitotecnia e Ciências Ambientais, Universidade Federal da Paraíba, Areia, Paraíba, Brazil.ORCID http://orcid.org/0000-0003-3096-6992
Divan Soares da SilvaDepartamento de Zootecnia, Universidade Federal da Paraíba, Areia, Paraíba, Brazil.ORCID http://orcid.org/0000-0003-0742-8440
José Ribamar Silva do Nascimento JúniorDepartamento de Zootecnia, Universidade Federal da Paraíba, Areia, Paraíba, Brazil.ORCID http://orcid.org/0000-0002-6150-5243
Fernando Dos Santos AraújoUniversidade Federal do Agreste de Pernambuco, Av. Bom Pastor s/n - Boa Vista, Garanhuns, Pernambuco, 55292-270, Brazil. fernandoaraujoagro177@gmail.com.ORCID http://orcid.org/0000-0002-0605-1613
Roberta de Lima ValençaUniversidade Federal do Agreste de Pernambuco, Av. Bom Pastor s/n - Boa Vista, Garanhuns, Pernambuco, 55292-270, Brazil.ORCID http://orcid.org/0000-0003-1341-1602
Elizanilda Ramalho do RêgoDepartamento de Ciências Fundamentais e Sociais, Universidade Federal da Paraíba, Areia, Paraíba, Brazil.ORCID http://orcid.org/0000-0001-7376-7569
Angela Maria Dos Santos PessoaUniversidade Federal do Ceará, Fortaleza, Ceará, Brazil.ORCID http://orcid.org/0000-0002-7393-984X
Riselane de Lucena Alcântara BrunoDepartamento de Fitotecnia e Ciências Ambientais, Universidade Federal da Paraíba, Areia, Paraíba, Brazil.ORCID http://orcid.org/0000-0001-7444-8543
Ariosvaldo Nunes de MedeirosDepartamento de Zootecnia, Universidade Federal da Paraíba, Areia, Paraíba, Brazil.ORCID http://orcid.org/0000-0002-1997-2649
Universidade Federal da Paraíba · BRUniversidade Federal de Pernambuco · BRUniversidade Federal do Agreste de PernambucoUniversidade Federal do Ceará · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDactylopius opuntiae (Cockerell) (carmine cochineal) is an insect pest highly noxious that has spread through cactus pear crops in the Brazilian semiarid region. Knowledge of diversity and genetic relationships of the cactus pear accessions is fundamental to create new varieties resistance to carmine cochineal. Therefore, this investigation was undertaken to assess the genetic diversity and genetic relationships that existed among cactus pear accessions of Nopalea sp. and Opuntia spp. with contrasting resistance to D. opuntiae. METHODS AND

resultsWe conducted a molecular analysis in seven cactus pear accessions from the "reference collection" of the Agronomic Institute of Pernambuco, Brazil using RAPD, ISSR and ITS molecular markers. A total of 242 bands were detected from 26 polymorphic primers. The high percentage of polymorphism by RAPD (89.8%), ISSR (81.2%) and ITS (75%) markers suggests that the cactus pear accessions have high genetic diversity. The combined analysis of markers systems enabled the accessions discrimination of about the genus and ploidy, but were incongruous in relation to resistance level to D. opuntiae.

conclusionsGenetic diversity, discrimination of about the genus and ploidy was confirmed by merging information from ISSR, RAPD and ITS markers systems. The IPA-200016, IPA-200149, IPA-100004, IPA-200205 accessions are genetically divergent, therefore could be potentially incorporated into any further breeding programs directed to create new varieties of cactus pear resistant to D. opuntiae.

Indexed as

OpuntiaBiomarkersCarmineGenetic VariationMicrosatellite RepeatsPhylogenyPlant BreedingRandom Amplified Polymorphic DNA TechniqueBiomarkersCarmineCactus pearCarmine cochinealPlant breedingSemi-arid

Identifiers

PMID35394600
OpenAlexW4225355833

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.