ArticleG3 (Bethesda, Md.)2022
A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles.
Article in G3 (Bethesda, Md.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 81 citations in OpenAlex.
- Finding the perfect promoter for Cas9 in homing gene drives using single cell transcriptome data.Nature communications · 2026Article
- Engineering resilient gene drives for sustainable malaria control by predicting, testing and overcoming target site resistance in Anopheles gambiae.PLoS biology · 2026Article
- Self-limiting population suppression gene drive design in the West Nile vector mosquito, Culex quinquefasciatus.Nature communications · 2026Article
- A simulation-based deep learning framework for spatially explicit malaria modeling of CRISPR suppression gene drive mosquitoes.iScience · 2026Article
- Experimental and modeling demonstration of stronger population suppression by gene drive targeting doublesex from dominant female-sterile resistance alleles.Nature communications · 2026Article
- Seed dormancy shapes gene drive dynamics in plants.Nature plants · 2026Article
- Advances in CRISPR gene drives for mosquito population control.Current opinion in microbiology · 2026Review
- Assessing target genes for homing suppression gene drive.The EMBO journal · 2026Article
- Strategies to improve the efficiency of homing gene drives with multiplexed gRNAs.BMC biology · 2025Article
- Modeling Control of Invasive Fire Ants by Gene Drive.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A male-drive female-sterile system for the self-limited control of the malaria mosquito Anopheles gambiae.Nature communications · 2025Article
- The Spatial Spread and the Persistence of Gene Drives Are Affected by Demographic Feedbacks, Density Dependence and Allee Effects.Molecular ecology · 2025Article
- Maximising Eradication Potential of Rat Gene Drives Using a Two-Target Homing Rescue Strategy: Spatial Modelling of Empirical Data.Molecular ecology · 2025Article
- Optimization of SgRNA expression with RNA pol III regulatory elements in Anopheles stephensi.Scientific reports · 2025Article
- A Comparative Assessment of Self-limiting Genetic Control Strategies for Population Suppression.Molecular biology and evolution · 2025Article
- Engineering drive-selection balance for localized population suppression with neutral dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Gene drive-based population suppression in the malaria vector Anopheles stephensi.Nature communications · 2025Article
- Repeat mediated excision of gene drive elements for restoring wild-type populations.PLoS genetics · 2024Article
- Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene.Nature communications · 2024Article
- The potential of gene drives in malaria vector species to control malaria in African environments.Nature communications · 2024Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Gene drives are engineered alleles that can bias inheritance in their favor, allowing them to spread throughout a population. They could potentially be used to modify or suppress pest populations, such as mosquitoes that spread diseases. CRISPR/Cas9 homing drives, which copy themselves by homology-directed repair in drive/wild-type heterozygotes, are a powerful form of gene drive, but they are vulnerable to resistance alleles that preserve the function of their target gene. Such resistance alleles can prevent successful population suppression. Here, we constructed a homing suppression drive in Drosophila melanogaster that utilized multiplexed gRNAs to inhibit the formation of functional resistance alleles in its female fertility target gene. The selected gRNA target sites were close together, preventing reduction in drive conversion efficiency. The construct reached a moderate equilibrium frequency in cage populations without apparent formation of resistance alleles. However, a moderate fitness cost prevented elimination of the cage population, showing the importance of using highly efficient drives in a suppression strategy, even if resistance can be addressed. Nevertheless, our results experimentally demonstrate the viability of the multiplexed gRNAs strategy in homing suppression gene drives.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.