ArticleNature communications2022
MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 117 papers, 1 of them a synthesis that pooled it.
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Who cites it
117 citing papers in PubMed, 1 synthesis or guideline pooled it, 368 citations in OpenAlex.
- The microbial dark matter and "wanted list" in worldwide wastewater treatment plants.Microbiome · 2023Pooled it
- The COVID-19 pandemic influenced the temporal dynamics of antimicrobial resistance markers and bacterial community across urban wastewater treatment plants.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Application of antibiotics for the selective isolation of previously uncultured species from activated sludge.Microbiology spectrum · 2026Article
- Soil microbial ecology and microbiome-metabolite linkages improve understanding of ecosystem states along terrestrial-aquatic interfaces.FEMS microbiology ecology · 2026Article
- Transient cyanobacteria drive hydrostatic photogranulation but disappear during microbial succession.Biofilm · 2026Article
- Trace lanthanum activation drives deep biological phosphorus removal.Environmental science and ecotechnology · 2026Article
- Effects of nitrogen and phosphorous concentrations on PHA synthesis by PNSB enriched phototrophic mixed microbial culture.Bioprocess and biosystems engineering · 2026Article
- Candidatus Dermatophostum as a novel genus of polyphosphate-accumulating organisms for high-strength wastewater treatment.The ISME journal · 2026Article
- Sulfonamide resistance geneMicrobiology spectrum · 2026Article
- The anaerobic digestion microbiome is robust toward variation in the waste activated sludge feed.ISME communications · 2026Article
- The role of bacteria in wastewater treatment and the impact of treated wastewater on riverine bacterial ecosystems.PloS one · 2026Article
- Revised 16S rRNA V4 hypervariable region targeting primers enhance detection of Patescibacteria and other lineages across diverse environments.ISME communications · 2026Article
- Bridging taxonomic gaps in microbial community profiling with LSTM-generated synthetic full-length 16S rRNA sequences.ISME communications · 2026Article
- Article
- Advances in the detection of antimicrobial resistance in aquatic environments: a methodological perspective.Biology methods & protocols · 2026Review
- Functional diversity in bacterial communities of an integrated constructed wetland used forFrontiers in microbiology · 2026Article
- Creating unity: linking 16S rRNA gene sequence information to the core taxonomy of genomes.Environmental microbiome · 2025Article
- Predicting microbial community structure and temporal dynamics by using graph neural network models.Nature communications · 2025Article
- Article
- Harnessing Engineered Microbial Consortia for Xenobiotic Bioremediation: Integrating Multi-Omics and AI for Next-Generation Wastewater Treatment.Journal of xenobiotics · 2025Review
57 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 20 institutions in 30 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the 'MiDAS 4' database. MiDAS 4 is an amplicon sequence variant resolved, full-length 16S rRNA gene reference database with a comprehensive taxonomy from domain to species level for all sequences. We use an independent dataset (269 WWTPs) to show that MiDAS 4, compared to commonly used universal reference databases, provides a better coverage for WWTP bacteria and an improved rate of genus and species level classification. Taking advantage of MiDAS 4, we carry out an amplicon-based, global-scale microbial community profiling of activated sludge plants using two common sets of primers targeting regions of the 16S rRNA gene, revealing how environmental conditions and biogeography shape the activated sludge microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 966 genera and 1530 species that represent approximately 80% and 50% of the accumulated read abundance, respectively. Finally, we show that for well-studied functional guilds, such as nitrifiers or polyphosphate-accumulating organisms, the same genera are prevalent worldwide, with only a few abundant species in each genus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.