Evidence map›Paper›PMID 35389621›Full record

ArticleACS synthetic biology2022

Coupling a Live Cell Directed Evolution Assay with Coevolutionary Landscapes to Engineer an Improved Fluorescent Rhodopsin Chloride Sensor.

Hsichuan Chi, Qin Zhou, Jasmine N Tutol, Shelby M Phelps, Jessica Lee, Paarth Kapadia, Faruck Morcos, Sheel C Dodani

Abstract read
In one paragraph

Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hsichuan Chi
Qin Zhou
Jasmine N Tutol
Shelby M Phelps
Jessica Lee
Paarth Kapadia
Faruck Morcos

Funding

Optical Imaging Tools for Elucidating the Roles of Anions and Anionic Modifications in Cellular SignalingR35GM128923 · NIGMS · UNIVERSITY OF TEXAS DALLAS · PI Sheel Dodani · 2018 to 2026
$3.4M
Computational Tools to Characterize the Effects of Protein and RNA Variability in Function and InteractionsR35GM133631 · NIGMS · UNIVERSITY OF TEXAS DALLAS · PI Alonso Faruck Morcos · 2019 to 2026
$3.0M
NIGMS NIH HHS R35 GM128923NIGMS NIH HHS R35 GM133631
6 · The paper itself

Abstract

Our understanding of chloride in biology has been accelerated through the application of fluorescent protein-based sensors in living cells. These sensors can be generated and diversified to have a range of properties using laboratory-guided evolution. Recently, we established that the fluorescent proton-pumping rhodopsin

Indexed as

ChloridesRhodopsinEscherichia coliProton PumpsProtonsChloridesProton PumpsProtonsRhodopsinchloridecoevolutiondirect coupling analysisfluorescent biosensorprotein engineeringrhodopsin

Identifiers

PMID35389621
PMCPMC9184236

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.