ArticleGenetics2022
Cas9-mediated maternal effect and derived resistance alleles in a gene-drive strain of the African malaria vector mosquito, Anopheles gambiae.
Article in Genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- Compound effector genes suppress malaria parasite infections in gene-drive population modification strains of the African malaria mosquitoes, Anopheles gambiae and Anopheles coluzzii.G3 (Bethesda, Md.) · 2026Article
- The nanosd integral gene drive enables population modification of the malaria vector Anopheles gambiae.G3 (Bethesda, Md.) · 2026Article
- Finding the perfect promoter for Cas9 in homing gene drives using single cell transcriptome data.Nature communications · 2026Article
- Long-term stability and performance of Cas9/guide RNA-based gene drives in anopheline mosquitoes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A simulation-based deep learning framework for spatially explicit malaria modeling of CRISPR suppression gene drive mosquitoes.iScience · 2026Article
- Article
- Engineering gene drive docking sites in a haplolethal locus in Anopheles gambiae.Scientific reports · 2025Article
- CRISPR technologies for the control and study of malaria-transmitting anopheline mosquitoes.Parasites & vectors · 2025Review
- Performance of two low-threshold population replacement gene drives in cage populations of the yellow fever mosquito, Aedes aegypti.PLoS genetics · 2025Article
- Challenges in developing a split drive targeting dsx for the genetic control of the invasive malaria vector Anopheles stephensi.Parasites & vectors · 2025Article
- Gene drives: an alternative approach to malaria control?Gene therapy · 2025Review
- Cas9/guide RNA-based gene-drive dynamics following introduction and introgression into diverse anopheline mosquito genetic backgrounds.BMC genomics · 2024Article
- Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene.Nature communications · 2024Article
- Article
- Next-generation CRISPR gene-drive systems using Cas12a nuclease.Nature communications · 2023Article
- Dual effector population modification gene-drive strains of the African malaria mosquitoes,Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Use of Insect Promoters in Genetic Engineering to Control Mosquito-Borne Diseases.Biomolecules · 2022Review
- Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito Aedes aegypti via single-generation crosses and modeling.G3 (Bethesda, Md.) · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9 technologies are important tools for the development of gene-drive systems to modify mosquito vector populations to control the transmission of pathogens that cause diseases such as malaria. However, one of the challenges for current Cas9-based drive systems is their ability to produce drive-resistant alleles resulting from insertions and deletions (indels) caused principally by nonhomologous end-joining following chromosome cleavage. Rapid increases in the frequency of such alleles may impair gene-drive dynamics. We explored the generation of indels in the germline and somatic cells in female gene-drive lineages using a series of selective crosses between a gene-drive line, AgNosCd-1, and wild-type mosquitoes. We find that potential drive-resistant mutant alleles are generated largely during embryonic development, most likely caused by deposition of the Cas9 endonuclease and guide RNAs in oocytes and resulting embryos by homozygous and hemizygous gene-drive mothers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.