Evidence map›Paper›PMID 35388225›Full record

ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2022

Validation of a competitive enzyme-linked immunosorbent assay to improve the serological diagnosis of swine vesicular disease.

Ming Yang, Leanne McIntyre, Wanhong Xu, Emiliana Brocchi, Santina Grazioli, Kathleen Hooper-McGrevy, Charles Nfon

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ming YangNational Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 (Yang, McIntyre, Xu, Hooper-McGrevy, Nfon); Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Via Bianchi, 9-25124 Brescia, Italy (Brocchi, Grazioli).
Leanne McIntyreNational Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 (Yang, McIntyre, Xu, Hooper-McGrevy, Nfon); Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Via Bianchi, 9-25124 Brescia, Italy (Brocchi, Grazioli).
Wanhong XuNational Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 (Yang, McIntyre, Xu, Hooper-McGrevy, Nfon); Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Via Bianchi, 9-25124 Brescia, Italy (Brocchi, Grazioli).
Emiliana BrocchiNational Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 (Yang, McIntyre, Xu, Hooper-McGrevy, Nfon); Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Via Bianchi, 9-25124 Brescia, Italy (Brocchi, Grazioli).
Santina GrazioliNational Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 (Yang, McIntyre, Xu, Hooper-McGrevy, Nfon); Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Via Bianchi, 9-25124 Brescia, Italy (Brocchi, Grazioli).
Kathleen Hooper-McGrevyNational Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 (Yang, McIntyre, Xu, Hooper-McGrevy, Nfon); Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Via Bianchi, 9-25124 Brescia, Italy (Brocchi, Grazioli).
Charles NfonNational Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 (Yang, McIntyre, Xu, Hooper-McGrevy, Nfon); Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Via Bianchi, 9-25124 Brescia, Italy (Brocchi, Grazioli).

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Swine vesicular disease (SVD) is an infectious viral disease of pigs. The clinical symptoms of SVD are indistinguishable from other vesicular diseases. In countries free of vesicular diseases, rapid SVD diagnosis and differentiation from other vesicular diseases are essential. In this report, a competitive enzyme-linked immunosorbent assay (cELISA) was developed and validated to improve the current SVD serological diagnosis. In this cELISA, an anti-SVD monoclonal antibody (mAb) captures the recombinant SVD virus-like particle (SVD-VLP) antigen, and 5B7 mAb is used as a competitor to compete with polyclonal antibodies in SVD-positive sera. The cut-off value of the SVD-VLP based cELISA (SVD-VLP cELISA) is ≥ 65% inhibition (%). The determined diagnostic specificity was 99.2%. SVD-VLP cELISA successfully detected SVD antibodies in the sera of SVD-infected animals and produced a diagnostic sensitivity of 100%. A panel of SVD positive sere including outbreak samples (

Indexed as

Swine DiseasesSwine Vesicular DiseaseAnimalsAntibodies, MonoclonalAntibodies, ViralEnzyme-Linked Immunosorbent AssaySensitivity and SpecificitySwineAntibodies, MonoclonalAntibodies, Viral

Identifiers

PMID35388225
PMCPMC8978281

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.