ArticleNature communications2022
Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
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- Installation of cationic phosphorus on dimethyldihydropyrene photoswitches: expanding the atomic repertoire towards far-red photoswitching in water.Chemical science · 2025Article
- Chip-Based Optoacoustic Single-Cell Detection in Flow Using Point-Source Optimized Surface Acoustic Wave Transducers.ACS applied materials & interfaces · 2025Article
- Article
- Impact of triplet state population on GFP-type fluorescence and photobleaching.Biology of the cell · 2025Article
- Genetically-encoded temperature indicators for thermal biology.Biophysics and physicobiology · 2025Article
- Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- High-affinity tuning of single fluorescent protein-type indicators by flexible linker length optimization in topology mutant.Communications biology · 2024Article
- Optical Phenomena in Molecule-Based Magnetic Materials.Chemical reviews · 2024Review
- Engineering of NEMO as calcium indicators with large dynamics and high sensitivity.Nature methods · 2023Article
- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Latest trends in bioimaging and building a proactive network of early-career young scientists around bioimaging in Europe.Biology open · 2022Article
- Quantitative Model for Reversibly Photoswitchable Sensors.ACS sensors · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically-encoded biosensors based on a single fluorescent protein are widely used to visualize analyte levels or enzymatic activities in cells, though usually to monitor relative changes rather than absolute values. We report photochromism-enabled absolute quantification (PEAQ) biosensing, a method that leverages the photochromic properties of biosensors to provide an absolute measure of the analyte concentration or activity. We develop proof-of-concept photochromic variants of the popular GCaMP family of Ca
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.