ArticleBMC plant biology2022
In vitro octaploid induction of Populus hopeiensis with colchicine.
Article in BMC plant biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 39 citations in OpenAlex.
- In vitro induction of tetraploids and their morphological and phenolic acid content in Cnidium officinale.BMC plant biology · 2026Article
- Creation of new varieties of tetraploid willow (Salix suchowensis) and their evaluation for salt tolerance.Molecular genetics and genomics : MGG · 2026Article
- Induction and phenotype analysis of autotetraploids of Morus Mongolica.BMC plant biology · 2026Article
- Comparative phenotypic and transcriptomic analyses reveal the potential molecular basis of forming bigger leaf blade in autotetraploid castor bean.BMC plant biology · 2025Article
- Colchicine-Induced Polyploidization Influences the Morphological, Physiological, and Biochemical Characteristics ofPlants (Basel, Switzerland) · 2025Article
- The Role of Colchicine in Plant Breeding.International journal of molecular sciences · 2025Review
- Article
- Salicylic acid modulates secondary metabolism and enhanced colchicine accumulation in long yellow daylily (AoB PLANTS · 2024Article
- Chromosome Doubling Enhances Biomass and Carotenoid Content inPlants (Basel, Switzerland) · 2024Article
- In vitro induction of tetraploidy and its effects on phenotypic variations in Populus hopeiensis.BMC plant biology · 2023Article
- Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method.Plant methods · 2023Article
- Article
- Differences in Leaf Morphology and Related Gene Expression between Diploid and Tetraploid Birch (International journal of molecular sciences · 2022Article
- Morphological, Transcriptome, and Hormone Analysis of Dwarfism in Tetraploids ofInternational journal of molecular sciences · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAutopolyploids, especially artificial lines, provide model systems for understanding the mechanisms of gene dosage effects on trait variation owing to their relatively uniform genetic background. Here, a protocol for in vitro octaploid induction of Populus hopeiensis from leaf blades with colchicine treatment was established through investigation of the effects of different pre-culture durations, colchicine concentrations, and exposure times.
resultsWe found that pre-culture duration, colchicine concentration, and exposure time had significant effects on the survival rate, shoot regeneration rate, and octaploid induction rate of P. hopeiensis leaf blades. The highest octaploid induction rate (8.61%) was observed when leaf blades pre-cultured for 9 days were treated for 4 days with 100 μM colchicine. The ploidy level of all regenerated plantlets was analyzed by flow cytometry and further confirmed by chromosome counting. A total of 14 octaploids were obtained. The stomatal length, width, and density of leaf blades significantly differed between tetraploid and octaploid plants. Compared with diploid and tetraploid plants, octaploids had a slower growth rate, smaller leaf blade size, and shorter internodes.
conclusionsWe established an effective protocol for inducing octaploids in vitro from autotetraploid P. hopeiensis leaf blades by colchicine treatment.
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