Evidence map›Paper›PMID 35386065›Full record

ArticleApplied biochemistry and biotechnology2022

Encapsulation of Nitrilase in Zeolitic Imidazolate Framework-90 to Improve Its Stability and Reusability.

Hui Peng, Wenge Dong, Qiwei Chen, Haiyan Song, Hongxu Sun, Ren Li, Yanhong Chang, Hui Luo

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hui PengDepartment of Environmental Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Wenge DongDepartment of Biological Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Qiwei ChenDepartment of Biological Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Haiyan SongDepartment of Environmental Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Hongxu SunDepartment of Biological Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Ren LiDepartment of Environmental Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Yanhong ChangDepartment of Environmental Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China. yhchang@ustb.edu.cn.
Hui LuoDepartment of Biological Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China. luohui@ustb.edu.cn.ORCID http://orcid.org/0000-0002-3075-2129

Funding

National Natural Science Foundation of China 22078019
6 · The paper itself

Abstract

In this study, nitrilase (Nit) was immobilized in zeolite imidazole framework-90 (ZIF-90) by one-pot biomimetic mineralization strategy. The structure, morphology and functional groups of ZIF-90 and immobilized enzyme Nit@ZIF-90 were characterized by scanning electron microscopy (SEM)/energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FT-IR). Circular dichroism (CD) proved that the immobilized method of encapsulation in ZIF-90 could effectively maintain the intrinsic conformation of Nit. Meanwhile, the stability and reusability of Nit@ZIF-90 were systematically evaluated. Compared with the free enzyme, the thermal, pH and organic solvents stability of Nit@ZIF-90 were significantly increased. Further, Nit@ZIF-90 exhibited better reusability during the hydrolysis of acrylonitrile and retained 48.34% of the initial activity after 10 cycles. Besides, the Ni@ZIF-90 had preferable storage stability, which showed a high degree of residual activity (more than 64 %) after storage at 4 °C for 7 d. The improved stability and reusability of the Nit@ZIF-90 implied that it could be used as a potential effective biocatalyst for hydrolysis of nitrile compounds in industrial application.

Indexed as

ZeolitesAminohydrolasesEnzymes, ImmobilizedSpectroscopy, Fourier Transform InfraredAminohydrolasesEnzymes, ImmobilizednitrilaseZeolitesbiomimetic mineralizationimmobilizationnitrilasereusabilitystabilityzeolite imidazole framework

Identifiers

PMID35386065

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.