Evidence map›Paper›PMID 35385528›Full record

ArticlePloS one2022

Effectiveness of treatment of bedding and feces of laboratory animal with ozone.

Jiao-Jiao Qiao, Shan-Ni Wang, Jing-Jing Li, Li-Yu Chen, Mei-Mei Wang, Bin Yi, Qing-Xia Liu, Yun-Bo Liu, Chen Zhang, Paul Honess and 1 more

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In one paragraph

Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jiao-Jiao QiaoDepartment of Clinical Laboratory, Xiang-Ya Hospital, Central South University, Changsha, China.
Shan-Ni WangDepartment of Clinical Laboratory, Xiang-Ya Hospital, Central South University, Changsha, China.
Jing-Jing LiDepartment of Clinical Laboratory, Xiang-Ya Hospital, Central South University, Changsha, China.
Li-Yu ChenDepartment of Microbiology, Xiang-Ya School of Medicine, Central South University, Changsha, China.
Mei-Mei WangDepartment of Clinical Laboratory, Xiang-Ya Hospital, Central South University, Changsha, China.
Bin YiDepartment of Clinical Laboratory, Xiang-Ya Hospital, Central South University, Changsha, China.
Qing-Xia LiuDepartment of Clinical Laboratory, Xiang-Ya Hospital, Central South University, Changsha, China.
Yun-Bo LiuInstitute of Laboratory Animal Sciences, CAMS & PUMC, Beijing, China.
Chen ZhangDepartment of Neurobiology, Beijing Key Laboratory of Neural Regeneration and Repair, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
Paul HonessSchool of Veterinary Medicine and Science, University of Nottingham, Nottingham, United Kingdom.
Chang-Qing GaoDepartment of Clinical Laboratory, Xiang-Ya Hospital, Central South University, Changsha, China.ORCID 0000-0001-6160-3807

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe incineration and burying of the soiled bedding of laboratory animals, as well as using detergents to treat their feces, is hazardous to the environment. This highlights the need for an alternative, environmentally friendly solution for the treatment of the waste of laboratory animal facilities. This study aims to evaluate the efficacy of ozone disinfection of the soiled bedding and feces of laboratory animals.

methodsTwo grams of soiled beddings were randomly sampled from the cages of mice and rats. These samples were mixed in a beaker with 40ml saline. Ozone was piped into the beaker at a concentration of 500mg/h. Samples were taken from the beaker at time 0min, 30min, 45min and 60min after ozone treatment for microbiological culturing in an incubator for 48h. Colony form unit of each plate (CFU/plate) at each time point were counted, the mean CFU/plate at each time point after ozone treatment were compared with that present at time zero. Feces of rabbits and dogs were treated and pathogens were counted the similar way as that of bedding of the mice and rats; samples being taken at 0min, 15min, 30min, 45min and 60min.

resultsPathogens were observed in beddings of both mice and rats as well as in feces of rabbits and dogs. Ozone treatment for 30min killed more than 93% of pathogens in the bedding of the two rodent species and 60min of treatment killed over 99% of pathogens. Treatment of rabbit and dog feces for 30min killed over 96% pathogens present, and 60min's treatment killed nearly all the pathogens. Both Gram positive and Gram negative pathogens were sensitive to ozone treatment.

conclusionOzone treatment of bedding and feces is an effective and environment friendly way to deal with the waste of animal facilities, saving energy and potentially enabling their reuse as fertilizer.

Indexed as

OzoneAnimalsAnimals, LaboratoryBedding and LinensDogsFecesHousing, AnimalRabbitsRatsOzone

Identifiers

PMID35385528
PMCPMC8985978

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.