ArticleAngewandte Chemie (International ed. in English)2022
Protocellular CRISPR/Cas-Based Diffusive Communication Using Transcriptional RNA Signaling.
Article in Angewandte Chemie (International ed. in English), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Bottom-Up Coacervate-Based Artificial Cells: Integrating Cellular Hallmarks into Complex Life-Like Systems.Angewandte Chemie (International ed. in English) · 2026Review
- RNA-Based Communication in Heterogeneous Populations of Cell Mimics.ACS synthetic biology · 2026Article
- Photoactivatable Synthetic Exosomes for RNA-Based Communication Between Artificial Cells and Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Strategies and applications of synthetic cell communication.Nature chemical biology · 2025Review
- Dynamic Transcription Machineries in Protocells.Journal of the American Chemical Society · 2025Review
- Photochemically Triggered, Transient, and Oscillatory Transcription Machineries Guide Temporal Modulation of Fibrinogenesis.Journal of the American Chemical Society · 2025Article
- Light-Based Juxtacrine Signaling Between Synthetic Cells.Small science · 2025Article
- Chemical Communication between Giant Vesicles and Gated Nanoparticles for Strip-Based Sensing.Nano letters · 2024Article
- Developmental assembly of multi-component polymer systems through interconnected synthetic gene networks in vitro.Nature communications · 2024Article
- Circular single-stranded DNA as a programmable vector for gene regulation in cell-free protein expression systems.Nature communications · 2024Article
- Models of Chemical Communication for Micro/Nanoparticles.Accounts of chemical research · 2024Article
- DNA as a universal chemical substrate for computing and data storage.Nature reviews. Chemistry · 2024Review
- Cell Free Expression in Proteinosomes Prepared from Native Protein-PNIPAAm Conjugates.Macromolecular bioscience · 2024Article
- DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access.Nature nanotechnology · 2023Article
- Autonomic Integration in Nested Protocell Communities.Journal of the American Chemical Society · 2023Article
- Artificial receptor-mediated phototransduction toward protocellular subcompartmentalization and signaling-encoded logic gates.Science advances · 2023Article
- Protocellular CRISPR/Cas-Based Diffusive Communication Using Transcriptional RNA Signaling.Angewandte Chemie (International ed. in English) · 2022Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protocells containing enzyme-driven biomolecular circuits that can process and exchange information offer a promising approach for mimicking cellular features and developing molecular information platforms. Here, we employ synthetic transcriptional circuits together with CRISPR/Cas-based DNA processing inside semipermeable protein-polymer microcompartments. We first establish a transcriptional protocell that can be activated by external DNA strands and produce functional RNA aptamers. Subsequently, we engineer a transcriptional module to generate RNA strands functioning as diffusive signals that can be sensed by neighboring protocells and trigger the activation of internalized DNA probes or localization of Cas nucleases. Our results highlight the opportunities to combine CRISPR/Cas machinery and DNA nanotechnology for protocellular communication and provide a step towards the development of protocells capable of distributed molecular information processing.
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