ArticlePlant physiology2022
PDAT regulates PE as transient carbon sink alternative to triacylglycerol in Nannochloropsis.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Triacylglycerol synthesis in microalgae under abiotic stress: an updated integrative review.Plant & cell physiology · 2026Review
- Recent research progress on the effects of microalgae on lipid metabolism in ruminants: A review of the gastrointestinal tract-brain axis.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Review
- Epitranscriptomic reprogramming in response to low COThe Plant journal : for cell and molecular biology · 2025Article
- Marine Microalgae-Microorganism Co-Cultures: An Insight intoFoods (Basel, Switzerland) · 2025Review
- Transcriptome analysis of lipid biosynthesis during kernel development in two walnut (Juglans regia L.) varieties of 'Xilin 3' and 'Xiangling'.BMC plant biology · 2024Article
- Towards Lipid from Microalgae: Products, Biosynthesis, and Genetic Engineering.Life (Basel, Switzerland) · 2024Review
- Review
- Transcriptional insights into Chlorella sp. ABC-001: a comparative study of carbon fixation and lipid synthesis under different COBiotechnology for biofuels and bioproducts · 2023Article
- Integrative lipidomics profile uncovers the mechanisms underlying high-level α-linolenic acid accumulation inHorticulture research · 2023Article
- Physiochemical and molecular responses of the diatom Phaeodactylum tricornutum to illumination transitions.Biotechnology for biofuels and bioproducts · 2023Article
- Zeaxanthin epoxidase is involved in the carotenoid biosynthesis and light-dependent growth of the marine alga Nannochloropsis oceanica.Biotechnology for biofuels and bioproducts · 2023Article
- Enhanced accumulation of oil through co-expression of fatty acid and ABC transporters in Chlamydomonas under standard growth conditions.Biotechnology for biofuels and bioproducts · 2022Article
- Lipid turnover and SQUAMOSA promoter-binding proteins mediate variation in fatty acid desaturation under early nitrogen deprivation revealed by lipidomic and transcriptomic analyses inFrontiers in plant science · 2022Article
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Triacylglycerols (TAGs) are the main storage lipids in photosynthetic organisms under stress. In the oleaginous alga Nannochloropsis oceanica, while multiple acyl CoA:diacylglycerol (DAG) acyltransferases (NoDGATs) are involved in TAG production, the role of the unique phospholipid:DAG acyltransferase (NoPDAT) remains unknown. Here, we performed a functional complementation assay in TAG-deficient yeast (Saccharomyces cerevisiae) and an in vitro assay to probe the acyltransferase activity of NoPDAT. Subcellular localization, overexpression, and knockdown (KD) experiments were also conducted to elucidate the role of NoPDAT in N. oceanica. NoPDAT, residing at the outermost plastid membrane, does not phylogenetically fall into the clades of algae or plants and uses phosphatidylethanolamine (PE) and phosphatidylglycerol with 16:0, 16:1, and 18:1 at position sn-2 as acyl-donors in vivo. NoPDAT KD, not triggering any compensatory mechanism via DGATs, led to an ∼30% decrease of TAG content, accompanied by a vast accumulation of PEs rich in 16:0, 16:1, and 18:1 fatty acids (referred to as "LU-PE") that was positively associated with CO2 availability. We conclude that the NoPDAT pathway is parallel to and independent of the NoDGAT pathway for oil production. LU-PE can serve as an alternative carbon sink for photosynthetically assimilated carbon in N. oceanica when PDAT-mediated TAG biosynthesis is compromised or under stress in the presence of high CO2 levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.