ArticleInternational endodontic journal2022
In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi-species biofilm community.
Article in International endodontic journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
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Who cites it
12 citing papers in PubMed.
- Setting Characteristics, Solubility, Bioactivity and Interaction with Dentin of Four Calcium Silicate-Based Endodontic Sealers.Journal of functional biomaterials · 2026Article
- N-acyl homoserine lactone signaling modulates bacterial community associated with human dental plaque.NPJ biofilms and microbiomes · 2025Article
- Multispecies Biofilms Treated With Endodontic Sealers or Calcium Hydroxide: Antimicrobial Activity and Changes in Community Composition.International endodontic journal · 2025Article
- Could the sealer thickness affect the filling ability of bioceramic endodontic materials? Evaluation in a simulated physiological condition.Journal of conservative dentistry and endodontics · 2025Article
- Microstructural and elemental characterization of novel bioactive glass bioceramic sealer using Fourier transform infrared and X-ray diffraction analysis.Journal of conservative dentistry and endodontics · 2025Article
- Physicochemical properties and biological interaction of calcium silicate-based sealers - in vivo model.Clinical oral investigations · 2025Article
- Effects of fluid shear stress on oral biofilm formation and composition and the transcriptional response of Streptococcus gordonii.Molecular oral microbiology · 2024Article
- Antibiofilm Efficacy of Calcium Silicate-Based Endodontic Sealers.Materials (Basel, Switzerland) · 2024Article
- The effect of dental material type and masticatory forces on periodontitis-derived subgingival microbiomes.Biofilm · 2024Article
- A comparative study of biological properties of three root canal sealers.Clinical oral investigations · 2023Article
- In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi-species biofilm community.International endodontic journal · 2022Article
- Does the ultrasonic activation of calcium silicate-based sealers affect their physicochemical properties?Brazilian dental journalArticle
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
aimTo evaluate the physicochemical properties of five root canal sealers and assess their effect on an ex vivo dental plaque-derived polymicrobial community. METHODOLOGY: Dental plaque-derived microbial communities were exposed to the sealers (AH Plus [AHP], GuttaFlow Bioseal [GFB], Endoseal MTA [ESM], Bio-C sealer [BCS] and BioRoot RCS [BRR]) for 3, 6 and 18 h. The sealers' effect on the biofilm biomass and metabolic activity was quantified using crystal violet (CV) staining and MTT assay, respectively. Biofilm community composition and morphology were assessed by denaturing gradient gel electrophoresis (DGGE), 16S rRNA sequencing and scanning electron microscopy. The ISO6876:2012 specifications were followed to determine the setting time, radiopacity, flowability and solubility. Obturated acrylic teeth were used to assess the sealers' effect on pH. Surface chemical characterization was performed using SEM with coupled energy-dispersive spectroscopy. Data normality was assessed using the Shapiro-Wilk test. One-way anova and Tukey's tests were used to analyze data from setting time, radiopacity, flowability and solubility. Two-way anova and Dunnett's tests were used for the data analysis from CV, MTT and pH. 16S rRNA sequencing data were analyzed for alpha (Shannon index and Chao analysis) and beta diversity (Bray-Curtis dissimilarities). Differences in community composition were evaluated by analysis of similarity (p < .05).
resultsThe sealers significantly influenced microbial community composition and morphology. All sealers complied with ISO6876:2012 requirements for setting time, radiopacity and flowability. Although only AHP effectively reduced the biofilm biomass, all sealers, except BRR, reduced biofilm metabolic activity.
conclusionDespite adequate physical properties, none of the sealers tested prevented biofilm growth. Significant changes in community composition were observed. If observed in vivo, these changes could affect intracanal microbial survival, pathogenicity and treatment outcomes.
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Registered trials
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