ArticleScientific reports2022
Screening and validation of reference genes for qRT-PCR of bovine skeletal muscle-derived satellite cells.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 17 citations in OpenAlex.
- Copper Promotes Myogenic Differentiation of Bovine Skeletal Muscle-Derived Cells by Activating the HIF-1α/β-catenin Signaling Axis.Biological trace element research · 2026Article
- RPS27L Maintains Nucleolar Homeostasis to Regulate Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells.Animals : an open access journal from MDPI · 2026Article
- Transcriptome-Wide m6A Methylation Landscape of Longissimus Dorsi Muscle in Indigenous Guizhou Cattle.Life (Basel, Switzerland) · 2026Article
- Matrix- and Differentiation Stage-Dependent Variability of Reference Genes: Rethinking Validation Strategies in 3T3-L1 Adipogenic Models.International journal of molecular sciences · 2026Article
- Camellia oil supplementation specifically enhances marbling scores via PI3K/Akt pathway while optimizing n-6/n-3 fatty acid ratios: A dual-benefit strategy for premium functional beef production.Food chemistry: X · 2026Article
- Ultrasound-guided skeletal muscle biopsy technique permits measurement of structural, functional, cellular and biochemical properties.Scientific reports · 2026Article
- The Effect of IL-17A and Combined Mechanical Injury on Meniscal Tissue Integrity In Vitro.Journal of clinical medicine · 2025Article
- Characterization of Gene Expression Suppression by Bovine Coronavirus Non-Structural Protein 1.Viruses · 2025Article
- Dietary L-glutamine supplementation enhances growth performance and carcass characteristics in Hanwoo heifers via hepatic and skeletal muscle gene regulation.Journal of animal science · 2025Article
- Evaluation of Reference Gene Stability in Goat Skeletal Muscle Satellite Cells during Proliferation and Differentiation Phases.Animals : an open access journal from MDPI · 2024Article
- A novel quantitative real-time PCR with theVeterinarni medicina · 2024Article
- An Enzyme-Free Method for Isolation and Expansion of Muscle Stem Cells for Cultivated Meat Applications.Methods in molecular biology (Clifton, N.J.) · 2024Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The accuracy of sixteen commonly used internal reference genes was assessed in skeletal muscle-derived satellite cells of Qinchuan cattle at different stages of proliferation and induction of differentiation to determine the most suitable ones. Quantitative real-time PCR and three commonly used algorithmic programs, GeNorm, NormFinder and BestKeeper, were used to evaluate the stability of expression of the candidate internal reference genes (GAPDH, ACTB, PPIA, LRP10, HPRT1, YWHAZ, B2M, TBP, EIF3K , RPS9, UXT, 18S rRNA, RPLP0, MARVELD, EMD and RPS15A) in skeletal muscle-derived satellite cells at 0, 12, 24, 36 and 48 h of growth and after differentiation for 0, 2, 4, 6 and 8 days. The expression of two satellite cell marker genes, CCNA2 and MYF5, was used for validation analysis. The results of the software analyses showed that GAPDH and RPS15A were the most stable reference gene combinations during in vitro proliferation of bovine skeletal muscle-derived satellite cells, RPS15A and RPS9 were the most stable reference gene combinations during in vitro induction of differentiation of the cells, and PPIA was the least stable reference gene during proliferation and differentiation and was not recommended. This study lays the foundation for the selection of reference genes for qRT-PCR during the proliferation and induction of differentiation of bovine skeletal muscle-derived satellite cells.
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