ArticlePlant cell reports2022
miR171 modulates induction of somatic embryogenesis in citrus callus.
Article in Plant cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Boosting genome editing in perennial plants by CRISPR-Combo mediated morphogenic gene activation.Nature communications · 2026Article
- Identification of Target Gene and Interacting Protein of TwoPlants (Basel, Switzerland) · 2024Article
- Dynamic temporal transcriptome analysis reveals grape VlMYB59-Horticulture research · 2024Article
- Modulation of Plant MicroRNA Expression: Its Potential Usability in Wheat (Current genomics · 2023Review
- Uncovering the miRNA-mediated regulatory network involved in Ma bamboo (Horticulture research · 2023Article
- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
- Article
- Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology.Plants (Basel, Switzerland) · 2023Review
- Role of microRNA miR171 in plant development.PeerJ · 2023Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
key messageOverexpression of miR171 restored SE competence in the recalcitrant citrus callus, and inhibition of miR171 function weakened SE competence in the strongly embryogenic citrus callus. Somatic embryogenesis (SE) is an important way of in vitro regeneration for plants. For perennial woody crops such as citrus, embryogenic callus is usually induced from unfertilized aborted ovules and widely used in biotechnology aided breeding. However, SE capacity always declines in callus during subculture, which makes regeneration difficult and hinders the application of biotechnology. We previously found that miR171 may be a regulator of SE in citrus, based on the abundant expression of csi-miR171c in the embryogenic callus and during SE of citrus. Here, we report that miR171 promotes SE and is required for SE in citrus. Overexpression of miR171 restored SE competence in the recalcitrant callus of 'Guoqing No.1' Satsuma mandarin (G1), whereas inhibition of miR171 function by Short Tandem Target Mimic (STTM) weakened SE competence in the strongly embryogenic callus of 'Valencia' sweet orange (V). The comparative transcriptomic analysis in miR171 overexpressed callus line (OE) and the wild type callus (WT) indicated that overexpression of miR171 decreased the expression level of its SCARECROW-LIKE (CsSCL) targets, and activated stress response related biological processes and metabolic processes that are required for cell differentiation. However, CsSCLs were up-regulated in the OE callus during SE induction process, which activated the cell division and developmental processes that are required for embryogenesis progress. Our results validate the function of miR171 in regulation of SE and reveal the biological responses provoked by miR171 in citrus that may promote SE.
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