ArticleApplied and environmental microbiology2022
Detection of SARS-CoV-2 Variants Mu, Beta, Gamma, Lambda, Delta, Alpha, and Omicron in Wastewater Settled Solids Using Mutation-Specific Assays Is Associated with Regional Detection of Variants in Clinical Samples.
Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- A 1-year study on SARS-CoV-2 variant shifts in wastewater using dPCR: comparison with clinical and GISAID data.mSystems · 2025Article
- Article
- Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool.International journal of molecular sciences · 2024Article
- Crykey: Rapid identification of SARS-CoV-2 cryptic mutations in wastewater.Nature communications · 2024Article
- A scoping review of global SARS-CoV-2 wastewater-based epidemiology in light of COVID-19 pandemic.Heliyon · 2024Article
- Tracking SARS-CoV-2 variants of concern in wastewater: an assessment of nine computational tools using simulated genomic data.Microbial genomics · 2024Article
- Wastewater tiling amplicon sequencing in sentinel sites reveals longitudinal dynamics of SARS-CoV-2 variants prevalence.Water research X · 2024Article
- Crykey: Rapid Identification of SARS-CoV-2 Cryptic Mutations in Wastewater.medRxiv : the preprint server for health sciences · 2023Article
- The Protective Effect of Virus Capsids on RNA and DNA Virus Genomes in Wastewater.Environmental science & technology · 2023Article
- Quantification and Differentiation of SARS-CoV-2 Variants in Wastewater for Surveillance.Environment & health (Washington, D.C.) · 2023Article
- Monkeypox viral nucleic acids detected using both DNA and RNA extraction workflows.The Science of the total environment · 2023Article
- An improved method for determining frequency of multiple variants of SARS-CoV-2 in wastewater using qPCR assays.The Science of the total environment · 2023Article
- Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA.Scientific data · 2023Article
- Wastewater Genomic Surveillance Captures Early Detection of Omicron in Utah.Microbiology spectrum · 2023Article
- SARS-CoV-2 Variants Detection Strategies in Wastewater Samples Collected in the Bangkok Metropolitan Region.Viruses · 2023Article
- Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making.Life (Basel, Switzerland) · 2023Article
- Wastewater monitoring in tourist cities as potential sentinel sites for near real-time dynamics of imported SARS-CoV-2 variants.The Science of the total environment · 2023Article
- Delta SARS-CoV-2 variant is entirely substituted by the omicron variant during the fifth COVID-19 wave in Attica region.The Science of the total environment · 2023Article
- Comprehensive mutation profiling from wastewater in southern Germany extends evidence of circulating SARS-CoV-2 diversity beyond mutations characteristic for Omicron.FEMS microbes · 2023Article
- Expansion of wastewater-based disease surveillance to improve health equity in California's Central Valley: sequential shifts in case-to-wastewater and hospitalization-to-wastewater ratios.Frontiers in public health · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes in the circulation of SARS-CoV-2 variants of concern (VOCs) may require changes in the public health response to the COVID-19 pandemic, as they have the potential to evade vaccines and pharmaceutical interventions and may be more transmissive than other SARS-CoV-2 variants. As such, it is essential to track and prevent their spread in susceptible communities. We developed digital reverse transcription (RT)-PCR assays for mutations characteristic of VOCs and used them to quantify those mutations in samples of wastewater settled solids collected from a publicly owned treatment works (POTW) during different phases of the COVID-19 pandemic. Wastewater concentrations of single mutations characteristic of each VOC, normalized by the concentration of a conserved SARS-CoV-2 N gene, correlate with regional estimates of the proportion of clinical infections caused by each VOC. These results suggest that targeted RT-PCR assays can be used to detect variants circulating in communities and inform the public health response to the pandemic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.