ArticleProceedings of the National Academy of Sciences of the United States of America1986
Reflex splanchnic nerve stimulation increases levels of proenkephalin A mRNA and proenkephalin A-related peptides in the rat adrenal medulla.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 111 citations in OpenAlex.
- Role of the Adrenal Medulla in Hypoglycaemia-Associated Autonomic Failure-A Diabetic Perspective.Metabolites · 2024Review
- Basal and Stress-Induced Network Activity in the Adrenal MedullaFrontiers in endocrinology · 2022Article
- AgRP-Expressing Adrenal Chromaffin Cells Are Involved in the Sympathetic Response to Fasting.Endocrinology · 2017Article
- Article
- Is PACAP the major neurotransmitter for stress transduction at the adrenomedullary synapse?Journal of molecular neuroscience : MN · 2012Review
- PACAP-cytokine interactions govern adrenal neuropeptide biosynthesis after systemic administration of LPS.Neuropharmacology · 2010Article
- Regulation of the biosynthesis of large dense-core vesicles in chromaffin cells and neurons.Cellular and molecular neurobiology · 1998Review
- The transcriptional regulation of the preproenkephalin gene.The Biochemical journal · 1995Review
- The adrenal chromaffin granule: a model for large dense core vesicles of endocrine and nervous tissue.Journal of anatomy · 1993Review
- The chromogranins A and B: the first 25 years and future perspectives.Neuroscience · 1992Review
- Regulation of proenkephalin A gene expression in aggregating fetal rat brain cells.Cellular and molecular neurobiology · 1991Article
- Co-localization of chromogranin A and B, secretogranin II and neuropeptide Y in chromaffin granules of rat adrenal medulla studied by electron microscopic immunocytochemistry.Histochemistry · 1989Article
- Proteins bound at adjacent DNA elements act synergistically to regulate human proenkephalin cAMP inducible transcription.The EMBO journal · 1988Article
- Neural and humoral factors separately regulate neuropeptide Y, enkephalin, and chromogranin A and B mRNA levels in rat adrenal medulla.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of reflex splanchnic nerve stimulation on proenkephalin A biosynthesis was investigated in the rat adrenal medulla. Tissue levels of native [Met5]enkephalin-like immunoreactivity (IR) (measured by direct RIA of tissue extracts), cryptic [Met5]enkephalin-like IR (calculated as the increase in [Met5]enkephalin-like IR detected in tissue extracts after sequential digestion with trypsin and carboxypeptidase B), and proenkephalin A mRNA were determined in adrenal medulla from rats sacrificed at various times after a period of insulin-induced hypoglycemia. Two hours of insulin hypoglycemia, which produced intense reflex stimulation of the splanchnic nerves as evidenced by a 55% decrease in the adrenal medulla catecholamine levels, resulted in a 3-fold increase in proenkephalin A mRNA levels in this tissue. The proenkephalin A mRNA levels reached a maximum 15-fold increase over control values 24 hr after this period of hypoglycemic stress and then gradually declined with an approximate half-life of 4 days. Native and cryptic [Met5]enkephalin-like IR had increased 9-fold and 12-fold, respectively, 24 hr after this period of hypoglycemia, and both demonstrated maximum increases of 130-fold and 50-fold, respectively, after 96 hr. Combined pretreatment (i.p. administration) with the ganglionic and muscarinic blocking agents chlorisondamine (5 mg/kg of body weight) and atropine (1 mg/kg) blocked the increase in levels of proenkephalin A mRNA seen in the rat adrenal medulla following insulin hypoglycemia. These data indicate that reflex splanchnic nerve discharge stimulates proenkephalin biosynthesis, probably at the level of gene expression.
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