ArticleScientific reports2022
Improved fluorescent phytochromes for in situ imaging.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Using Computational Techniques for the Characterization of Structural Changes During the Phytochrome Photocycle.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Circular dichroism spectroscopy reveals multiple phytochrome photoproducts in equilibrium.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- Pr and Pfr structures of plant phytochrome A.Nature communications · 2025Article
- Nonlinear optical properties of photosensory core modules of monomeric and dimeric bacterial phytochromes.Protein science : a publication of the Protein Society · 2025Article
- Integrated Study of Fluorescence Enhancement in the Y176H Variant of Cyanobacterial Phytochrome Cph1.Biochemistry · 2025Article
- Outstanding women scientists who have broadened the knowledge on biological photoreceptors-II.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- Visualization of Nanocarriers and Drugs in Cells and Tissue.Handbook of experimental pharmacology · 2024Article
- Osmolytes Modulate Photoactivation of Phytochrome: Probing Protein Hydration.Molecules (Basel, Switzerland) · 2023Article
- Insights into the Structures of Bilirubin and Biliverdin from Vibrational and Electronic Circular Dichroism: History and Perspectives.Molecules (Basel, Switzerland) · 2023Review
- Long-Distance Protonation-Conformation Coupling in Phytochrome Species.Molecules (Basel, Switzerland) · 2022Article
- Protein control of photochemistry and transient intermediates in phytochromes.Nature communications · 2022Article
- Conserved histidine and tyrosine determine spectral responses through the water network in Deinococcus radiodurans phytochrome.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modern biology investigations on phytochromes as near-infrared fluorescent pigments pave the way for the development of new biosensors, as well as for optogenetics and in vivo imaging tools. Recently, near-infrared fluorescent proteins (NIR-FPs) engineered from biliverdin-binding bacteriophytochromes and cyanobacteriochromes, and from phycocyanobilin-binding cyanobacterial phytochromes have become promising probes for fluorescence microscopy and in vivo imaging. However, current NIR-FPs typically suffer from low fluorescence quantum yields and short fluorescence lifetimes. Here, we applied the rational approach of combining mutations known to enhance fluorescence in the cyanobacterial phytochrome Cph1 to derive a series of highly fluorescent variants with fluorescence quantum yield exceeding 15%. These variants were characterised by biochemical and spectroscopic methods, including time-resolved fluorescence spectroscopy. We show that these new NIR-FPs exhibit high fluorescence quantum yields and long fluorescence lifetimes, contributing to their bright fluorescence, and provide fluorescence lifetime imaging measurements in E.coli cells.
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