ArticleMethods in enzymology2022
Utilizing imaging mass spectrometry to analyze microbial biofilm chemical responses to exogenous compounds.
Article in Methods in enzymology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 10 citations in OpenAlex.
- Applications and technical challenges of MALDI mass spectrometry imaging in microbial ecosystems and metabolic network research.Archives of microbiology · 2026Review
- Lipopeptides and antibiotics from a marinemSystems · 2026Article
- The Future of a Myriad of Accelerated Biodiscoveries Lies in AI-Powered Mass Spectrometry and Multiomics Integration.Journal of mass spectrometry : JMS · 2025Article
- Molecular imaging uncovers growth media influence on biofilms' EPS production.Frontiers in chemistry · 2025Article
- Rapid and Robust Workflows Using Different Ionization, Computation, and Visualization Approaches for Spatial Metabolome Profiling of Microbial Natural Products inACS measurement science au · 2024Article
- Advances in methods and concepts provide new insight into antibiotic fluxes across the bacterial membrane.Communications biology · 2024Article
- A Label-Free Approach for Relative Spatial Quantitation of c-di-GMP in Microbial Biofilms.Analytical chemistry · 2024Article
- Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.Methods (San Diego, Calif.) · 2024Review
- ReducingAnalytical chemistry · 2023Article
- A label-free approach for relative spatial quantitation of c-di-GMP in microbial biofilms.bioRxiv : the preprint server for biology · 2023Article
- Multi-omics characterization of the microbial populations and chemical space composition of a water kefir fermentation.Frontiers in molecular biosciences · 2023Article
- Imaging mass spectrometry reveals complex lipid distributions acrossJournal of mass spectrometry and advances in the clinical lab · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is an appealing label-free method for imaging biological samples which focuses on the spatial distribution of chemical signals. This approach has been used to study the chemical ecology of microbes and can be applied to study the chemical responses of microbes to treatment with exogenous compounds. Specific conjugated cholic acids such as taurocholic acid (TCA), have been shown to inhibit biofilm formation in the enteric pathogen Vibrio cholerae and MALDI-IMS can be used to directly observe the chemical responses of V. cholerae biofilm colonies to treatment with TCA. A major challenge of MALDI-IMS is optimizing the sample preparation and drying for a particular growth condition and microbial strain. Here we demonstrate how V. cholerae is cultured and prepared for MALDI-IMS analysis and highlight critical steps to ensure proper sample adherence to a MALDI target plate and maintain spatial distributions when applying this technique to any microbial strain. We additionally show how to use both manual interrogation and statistical analyses of MALDI-IMS data to establish the adequacy of the sample preparation protocol. This protocol can serve as a guideline for the development of sample preparation techniques and the acquisition of high quality MALDI-IMS data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.