Evidence map›Paper›PMID 35379345›Full record

ArticleCell & bioscience2022

PA1 participates in the maintenance of blood-testis barrier integrity via cooperation with JUN in the Sertoli cells of mice.

Bo Liu, Chao Liu, Binfang Ma, Ruidan Zhang, Zhiwei Zhao, Sai Xiao, Wanjun Cao, Yanjie Ma, Guozhang Zhu, Wei Li and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell & bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Bo Liu *Department of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, Xi'an, 710032, China.
Chao Liu *Institute of Reproductive Health and Perinatology, Guangzhou Women and Children's Medical Center, Guangzhou, 510000, China.
Binfang Ma *Department of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, Xi'an, 710032, China.
Ruidan ZhangUniversity of the Chinese Academy of Sciences, Beijing, 100049, China.
Zhiwei ZhaoDepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, Xi'an, 710032, China.
Sai XiaoUniversity of the Chinese Academy of Sciences, Beijing, 100049, China.
Wanjun CaoDepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, Xi'an, 710032, China.
Yanjie MaInstitute of Reproductive Health and Perinatology, Guangzhou Women and Children's Medical Center, Guangzhou, 510000, China.
Guozhang ZhuDepartment of Biology, Marshall University, Huntington, WV, 25755, USA.
Wei LiInstitute of Reproductive Health and Perinatology, Guangzhou Women and Children's Medical Center, Guangzhou, 510000, China. leways@lzu.edu.cn.
Zhen LiDepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, Xi'an, 710032, China. lizhenhe@fmmu.edu.cn.
Air Force Medical University · CNGuangzhou Medical University · CNUniversity of Chinese Academy of Sciences · CNGuangzhou Women and Children Medical Center · CNMarshall University · US

Funding

National Natural Science Foundation of China 31871515National Natural Science Foundation of China 81925015
6 · The paper itself

Abstract

backgroundThe blood-testis barrier (BTB) is essential to the microenvironment of spermatogenesis, and Sertoli cells provide the cellular basis for BTB construction. Numerous nuclear transcription factors have been identified to be vital for the proper functioning of Sertoli cells. PA1 has been reported to play important roles during diverse biological processes, yet its potential function in male reproduction is still unknown.

resultsHere, we show that PA1 was highly expressed in human and mouse testis and predominantly localized in the nuclei of Sertoli cells. Sertoli cell-specific Pa1 knockout resulted in an azoospermia-like phenotype in mice. The knockout of this gene led to multiple defects in spermatogenesis, such as the disorganization of the cytoskeleton during basal and apical ectoplasmic specialization and the disruption of the BTB. Further transcriptomic analysis, together with Cut-Tag results of PA1 in Sertoli cells, revealed that PA1 could affect the expression of a subset of genes that are essential for the normal function of Sertoli cells, including those genes associated with actin organization and cellular junctions such as Connexin43 (Cx43). We further demonstrated that the expression of Cx43 depended on the interaction between JUN, one of the AP-1 complex transcription factors, and PA1.

conclusionOverall, our findings reveal that PA1 is essential for the maintenance of BTB integrity in Sertoli cells and regulates BTB construction-related gene expression via transcription factors. Thus, this newly discovered mechanism in Sertoli cells provides a potential diagnostic or even therapeutic target for some individuals with azoospermia.

Indexed as

Blood–testis-barrierConnexin43JUNPA1Sertoli cell

Identifiers

PMID35379345
PMCPMC8981650
OpenAlexW4226068414

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.