ArticleProceedings of the National Academy of Sciences of the United States of America2022
Single cell enhancer activity distinguishes GABAergic and cholinergic lineages in embryonic mouse basal ganglia.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Mesoscopic analysis of GABAergic marker expression in acetylcholine neurons in the whole mouse brain.iScience · 2026Article
- Single-cell sequencing of rodent ventral pallidum reveals diverse neuronal subtypes with noncanonical interregional continuity.Science advances · 2025Article
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- Persistent open chromatin state in early-life stress-activated cells of the VTA.Scientific reports · 2025Article
- Mesoscopic analysis of GABAergic marker expression in acetylcholine neurons in the whole mouse brain.bioRxiv : the preprint server for biology · 2025Article
- TEAD switches interacting partners along neural progenitor lineage progression to execute distinct functions.Genes & development · 2025Article
- CACNA1A loss-of-function affects neurogenesis in human iPSC-derived neural models.Cellular and molecular life sciences : CMLS · 2025Article
- Cross-species single-cell transcriptomics reveals neuronal similarities and heterogeneity in amniote pallium.Zoological research · 2025Article
- Conservation, alteration, and redistribution of mammalian striatal interneurons.bioRxiv : the preprint server for biology · 2024Article
- DLX genes and proteins in mammalian forebrain development.Development (Cambridge, England) · 2024Review
- Spatial enhancer activation influences inhibitory neuron identity during mouse embryonic development.Nature neuroscience · 2024Article
- Cell-type-specificeLife · 2024Article
- Patterning ganglionic eminences in developing human brain organoids using a morphogen-gradient-inducing device.Cell reports methods · 2024Article
- Critical test of the assumption that the hypothalamic entopeduncular nucleus of rodents is homologous with the primate internal pallidum.The Journal of comparative neurology · 2023Article
- The transcription regulator Lmo3 is required for the development of medial ganglionic eminence derived neurons in the external globus pallidus.Developmental biology · 2023Article
- Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy.Cell stem cell · 2023Article
- Basal forebrain cholinergic signalling: development, connectivity and roles in cognition.Nature reviews. Neuroscience · 2023Review
- The transcription factorFrontiers in cell and developmental biology · 2022Article
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17 authors.
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Abstract
Enhancers integrate transcription factor signaling pathways that drive cell fate specification in the developing brain. We paired enhancer labeling and single-cell RNA-sequencing (scRNA-seq) to delineate and distinguish specification of neuronal lineages in mouse medial, lateral, and caudal ganglionic eminences (MGE, LGE, and CGE) at embryonic day (E)11.5. We show that scRNA-seq clustering using transcription factors improves resolution of regional and developmental populations, and that enhancer activities identify specific and overlapping GE-derived neuronal populations. First, we mapped the activities of seven evolutionarily conserved brain enhancers at single-cell resolution in vivo, finding that the selected enhancers had diverse activities in specific progenitor and neuronal populations across the GEs. We then applied enhancer-based labeling, scRNA-seq, and analysis of in situ hybridization data to distinguish transcriptionally distinct and spatially defined subtypes of MGE-derived GABAergic and cholinergic projection neurons and interneurons. Our results map developmental origins and specification paths underlying neurogenesis in the embryonic basal ganglia and showcase the power of scRNA-seq combined with enhancer-based labeling to resolve the complex paths of neuronal specification underlying mouse brain development.
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