ArticleThe New phytologist2022
A new approach to zip-lignin: 3,4-dihydroxybenzoate is compatible with lignification.
Article in The New phytologist, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Engineering 2-Pyrone-4,6-Dicarboxylic Acid Production Reveals Metabolic Plasticity of Poplar.Plant biotechnology journal · 2026Article
- Diol-enhanced natural deep eutectic solvents for efficient poplar pretreatment.Frontiers in chemistry · 2026Article
- Engineered Accumulation of Protocatechuate in Corn Biomass to Enhance Biomanufacturing.ACS sustainable chemistry & engineering · 2025Article
- Genetically Engineered Poplar Wood Effectively Enhances the Efficiency of Deep Eutectic Solvent-Mediated One-Pot Processing.ChemSusChem · 2025Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- Suppression of Chorismate Mutase 1 in Hybrid Poplar to Investigate Potential Redundancy in the Supply of Lignin Precursors.Plant direct · 2025Article
- Advances in lignocellulosic feedstocks for bioenergy and bioproducts.Nature communications · 2025Review
- Synthetic-biology approach for plant lignocellulose engineering.Plant biotechnology (Tokyo, Japan) · 2024Article
- Expression of dehydroshikimate dehydratase in poplar induces transcriptional and metabolic changes in the phenylpropanoid pathway.Journal of experimental botany · 2024Article
- Enhancing monolignol ferulate conjugate levels in poplar lignin via OsFMT1.Biotechnology for biofuels and bioproducts · 2024Article
- EngineeringApplied and environmental microbiology · 2024Article
- Article
- Field performance of switchgrass plants engineered for reduced recalcitrance.Frontiers in plant science · 2023Article
- Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts.Biotechnology for biofuels and bioproducts · 2022Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- Engineering Curcumin Biosynthesis in Poplar Affects Lignification and Biomass Yield.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Renewed interests in the development of bioenergy, biochemicals, and biomaterials have elicited new strategies for engineering the lignin of biomass feedstock plants. This study shows, for the first time, that 3,4-dihydroxybenzoate (DHB) is compatible with the radical coupling reactions that assemble polymeric lignin in plants. We introduced a bacterial 3-dehydroshikimate dehydratase into hybrid poplar (Populus alba × grandidentata) to divert carbon flux away from the shikimate pathway, which lies upstream of lignin biosynthesis. Transgenic poplar wood had up to 33% less lignin with p-hydroxyphenyl units comprising as much as 10% of the lignin. Mild alkaline hydrolysis of transgenic wood released fewer ester-linked p-hydroxybenzoate groups than control trees, and revealed the novel incorporation of cell-wall-bound DHB, as well as glycosides of 3,4-dihydroxybenzoic acid (DHBA). Two-dimensional nuclear magnetic resonance (2D-NMR) analysis uncovered DHBA-derived benzodioxane structures suggesting that DHB moieties were integrated into the lignin polymer backbone. In addition, up to 40% more glucose was released from transgenic wood following ionic liquid pretreatment and enzymatic hydrolysis. This work highlights the potential of diverting carbon flux from the shikimate pathway for lignin engineering and describes a new type of 'zip-lignin' derived from the incorporation of DHB into poplar lignin.
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