Evidence map›Paper›PMID 35377194›Full record

ArticleEnvironmental health perspectives2022

Association of Glyphosate Exposure with Blood DNA Methylation in a Cross-Sectional Study of Postmenopausal Women.

Rachel M Lucia, Wei-Lin Huang, Khyatiben V Pathak, Marissa McGilvrey, Victoria David-Dirgo, Andrea Alvarez, Deborah Goodman, Irene Masunaka, Andrew O Odegaard, Argyrios Ziogas and 3 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental health perspectives, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
  2. Environmental Health Is Overlooked in Longevity Research.Antioxidants (Basel, Switzerland) · 2025
    Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Rachel M LuciaDepartment of Epidemiology and Biostatistics, University of California, Irvine, California, USA.
Wei-Lin HuangDepartment of Epidemiology and Biostatistics, University of California, Irvine, California, USA.
Khyatiben V PathakIntegrated Mass Spectrometry Shared Resource, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Marissa McGilvreyIntegrated Mass Spectrometry Shared Resource, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Victoria David-DirgoIntegrated Mass Spectrometry Shared Resource, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Andrea AlvarezDepartment of Medicine, University of California, Irvine, California, USA.
Deborah GoodmanDepartment of Epidemiology and Biostatistics, University of California, Irvine, California, USA.ORCID 0000-0003-2006-8718
Irene MasunakaDepartment of Medicine, University of California, Irvine, California, USA.ORCID 0000-0001-6740-3435
Andrew O OdegaardDepartment of Epidemiology and Biostatistics, University of California, Irvine, California, USA.
Argyrios ZiogasDepartment of Medicine, University of California, Irvine, California, USA.ORCID 0000-0003-4529-3727
Patrick PirrotteIntegrated Mass Spectrometry Shared Resource, City of Hope Comprehensive Cancer Center, Duarte, California, USA.ORCID 0000-0003-1360-6039
Trina M Norden-KrichmarDepartment of Epidemiology and Biostatistics, University of California, Irvine, California, USA.ORCID 0000-0002-0199-9915
Hannah Lui ParkDepartment of Epidemiology and Biostatistics, University of California, Irvine, California, USA.ORCID 0000-0001-9973-1396
University of California, Irvine · USTranslational Genomics Research Institute · US

Funding

Transgenic Mouse FacilityP30CA033572 · NCI · CITY OF HOPE/BECKMAN RESEARCH INSTITUTE · PI John Charles Williams · 1985 to 2026
$86.3M
NCI NIH HHS P30 CA033572
6 · The paper itself

Abstract

backgroundGlyphosate is the most commonly used herbicide in the world and is purported to have a variety of health effects, including endocrine disruption and an elevated risk of several types of cancer. Blood DNA methylation has been shown to be associated with many other environmental exposures, but to our knowledge, no studies to date have examined the association between blood DNA methylation and glyphosate exposure.

objectiveWe conducted an epigenome-wide association study to identify DNA methylation loci associated with urinary glyphosate and its metabolite aminomethylphosphonic acid (AMPA) levels. Secondary goals were to determine the association of epigenetic age acceleration with glyphosate and AMPA and develop blood DNA methylation indices to predict urinary glyphosate and AMPA levels.

methodsFor 392 postmenopausal women, white blood cell DNA methylation was measured using the Illumina Infinium MethylationEPIC BeadChip array. Glyphosate and AMPA were measured in two urine samples per participant using liquid chromatography-tandem mass spectrometry. Methylation differences at the probe and regional level associated with glyphosate and AMPA levels were assessed using a resampling-based approach. Probes and regions that had an false discovery rate

resultsWe identified 24 CpG sites whose methylation level was associated with urinary glyphosate concentration and two associated with AMPA. Four regions, within the promoters of the DISCUSSION: Glyphosate and AMPA exposure were associated with DNA methylation differences that could promote the development of cancer and other diseases. Further studies are warranted to replicate our results, determine the functional impact of glyphosate- and AMPA-associated differential DNA methylation, and further explore whether DNA methylation could serve as a biomarker of glyphosate exposure. https://doi.org/10.1289/EHP10174.

Indexed as

DNA MethylationPostmenopauseCross-Sectional StudiesDNA Repair EnzymesFemaleGlycineGlyphosateHumansKv1.6 Potassium ChannelTranscription FactorsDNA Repair EnzymesERCC8 protein, humanGlycineGlyphosateKCNA6 protein, humanKv1.6 Potassium ChannelTranscription Factors

Identifiers

PMID35377194
PMCPMC8978648
OpenAlexW4225525035

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.