Evidence map›Paper›PMID 35377166›Full record

ArticleJournal of bacteriology2022

Nonmotile Subpopulations of

Kimberley A Lewis, Danielle M Vermilyea, Shanice S Webster, Christopher J Geiger, Jaime de Anda, Gerard C L Wong, George A O'Toole, Deborah A Hogan

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. HowmBio · 2024
    Article
  5. Article
  6. Swarming ofBiophysics reviews · 2023
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Kimberley A Lewis *Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.
Danielle M Vermilyea *Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.ORCID 0000-0001-6147-5438
Shanice S WebsterDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.
Christopher J GeigerDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.
Jaime de AndaDepartment of Bioengineering, University of California Los Angeles, Los Angeles, California, USA.
Gerard C L WongDepartment of Bioengineering, University of California Los Angeles, Los Angeles, California, USA.
George A O'TooleDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.ORCID 0000-0002-2861-4392
Deborah A HoganDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.ORCID 0000-0002-6366-2971
Dartmouth College · USCalifornia NanoSystems Institute · US

Funding

Translational Engineering in Cancer (TEC)P30CA023108 · NCI · DARTMOUTH COLLEGE · PI Fred W Kolling IV · 1985 to 2026
$91.3M
Understanding the role of RNA-binding protein mutations in cancerP20GM113132 · NIGMS · DARTMOUTH COLLEGE · PI MADDEN, DEAN R · 2016 to 2025
$25.9M
Translational Research CoreP30DK117469 · NIDDK · DARTMOUTH COLLEGE · PI DEAN R MADDEN · 2018 to 2026
$13.9M
HOST-MICROBE INTERACTIONST32AI007519 · NIAID · DARTMOUTH COLLEGE · PI DEBORAH A HOGAN · 1997 to 2026
$5.9M
cdiGMP regulation of Biofilm FormationR37AI083256 · NIAID · DARTMOUTH COLLEGE · PI O'TOOLE, GEORGE A. · 2014 to 2023
$4.7M
Surface sensing, memory, and motility control in biofilm formationR01AI143730 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI O'TOOLE, GEORGE A., WONG, GERARD C · 2019 to 2023
$2.0M
NCI NIH HHS P30 CA023108NIAID NIH HHS R01 AI143730NIAID NIH HHS R37 AI083256NIAID NIH HHS T32 AI007519NIDDK NIH HHS P30 DK117469NIGMS NIH HHS P20 GM113132
6 · The paper itself

Abstract

The downregulation of Pseudomonas aeruginosa flagellar motility is a key event in biofilm formation, host colonization, and the formation of microbial communities, but the external factors that repress motility are not well understood. Here, we report that on soft agar, swarming motility can be repressed by cells that are nonmotile due to the absence of a flagellum or flagellar rotation. Mutants that lack either flagellum biosynthesis or rotation, when present at as little as 5% of the total population, suppressed swarming of wild-type cells. Non-swarming cells required functional type IV pili and the ability to produce Pel exopolysaccharide to suppress swarming by the flagellated wild type. Flagellated cells required only type IV pili, but not Pel production, for their swarming to be repressed by non-flagellated cells. We hypothesize that interactions between motile and nonmotile cells may enhance the formation of sessile communities, including those involving multiple genotypes, phenotypically diverse cells, and perhaps other species.

Indexed as

Gene Expression Regulation, BacterialPseudomonas aeruginosaBacterial ProteinsFimbriae, BacterialFlagellaBacterial Proteinsmicrobe-microbe interactionmotilityPelPseudomonas aeruginosaswarmingtype IV pili

Identifiers

PMID35377166
PMCPMC9112919
OpenAlexW4225639134

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.