Evidence map›Paper›PMID 35372810›Full record

ArticleiScience2022

High-resolution Slide-seqV2 spatial transcriptomics enables discovery of disease-specific cell neighborhoods and pathways.

Jamie L Marshall, Teia Noel, Qingbo S Wang, Haiqi Chen, Evan Murray, Ayshwarya Subramanian, Katherine A Vernon, Silvana Bazua-Valenti, Katie Liguori, Keith Keller and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Jamie L MarshallKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Teia NoelKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Qingbo S WangProgram in Medical and Population Genetics, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Haiqi ChenProgram in Cell Circuits and Epigenetics, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Evan MurrayStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Ayshwarya SubramanianKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Katherine A VernonKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Silvana Bazua-ValentiKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Katie LiguoriKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Keith KellerKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Robert R StickelsCecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Breanna McBeanBroad Summer Research Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Rowan M HeneghanKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Astrid WeinsDepartment of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
Evan Z MacoskoStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Fei ChenProgram in Cell Circuits and Epigenetics, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Anna GrekaKidney Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Funding

University of Michigan Training Program in Genomic ScienceT32HG000040 · NHGRI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Sebastian Zoellner · 1995 to 2026
$16.4M
Molecular mechanisms of podocyte injury in FSGSR01DK095045 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI Anna Greka, Juan Lorenzo Pablo · 2014 to 2026
$5.1M
Role of TRPC5 channel inhibition in the treatment of glomerular diseaseR01DK099465 · NIDDK · BROAD INSTITUTE, INC. · PI Anna Greka · 2014 to 2026
$5.1M
NHGRI NIH HHS T32 HG000040NIDDK NIH HHS R01 DK095045NIDDK NIH HHS R01 DK099465
6 · The paper itself

Abstract

High-resolution spatial transcriptomics enables mapping of RNA expression directly from intact tissue sections; however, its utility for the elucidation of disease processes and therapeutically actionable pathways remains unexplored. We applied Slide-seqV2 to mouse and human kidneys, in healthy and distinct disease paradigms. First, we established the feasibility of Slide-seqV2 in tissue from nine distinct human kidneys, which revealed a cell neighborhood centered around a population of

Indexed as

Cell biologyPathophysiologyTranscriptomics

Identifiers

PMID35372810
PMCPMC8971939

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.