ArticleFrontiers in cardiovascular medicine2022
Application of Metagenomic Next-Generation Sequencing in the Etiological Diagnosis of Infective Endocarditis During the Perioperative Period of Cardiac Surgery: A Prospective Cohort Study.
Article in Frontiers in cardiovascular medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 39 citations in OpenAlex.
- Metagenomic next-generation sequencing in blood culture-negative endocarditis: a structured review with illustrative pooled estimates.Infection · 2026Review
- Current Surgical Approaches to Managing Infective Endocarditis With Aortic Root Abscess.Reviews in cardiovascular medicine · 2026Review
- Intraoperative sampling for postoperative metagenomic next-generation sequencing to guide biofilm-targeted therapy forFrontiers in cardiovascular medicine · 2026Article
- Clinical Utility and Therapeutic Strategy Value of Metagenomic Next-Generation Sequencing in Pulmonary Infection Among Cancer Patients.Infection and drug resistance · 2026Article
- Application of metagenomic next-generation sequencing in gastrointestinal infections in children after allogeneic hematopoietic stem cell transplantation.Frontiers in cellular and infection microbiology · 2026Article
- 16S rRNA Metabarcoding Used to Identify the Etiology of Infective Endocarditis Agents.Sovremennye tekhnologii v meditsine · 2026Article
- Unveiling the Future of Infective Endocarditis Diagnosis: The Transformative Role of Metagenomic Next-Generation Sequencing in Culture-Negative Cases.Journal of epidemiology and global health · 2025Review
- Integrated plasma and vegetation proteomic characterization of infective endocarditis for early diagnosis and treatment.Nature communications · 2025Article
- From Culture-Negative to DNA-Positive: The Molecular Revolution in Infective Endocarditis Diagnosis.Pathogens (Basel, Switzerland) · 2025Review
- Infective endocarditis caused by Streptococcus sinensis diagnosed with next-generation sequencing: a case report and literature review.BMC infectious diseases · 2025Review
- The Evolving Landscape of Infective Endocarditis: Difficult-to-Treat Resistance Bacteria and Novel Diagnostics at the Foreground.Journal of clinical medicine · 2025Review
- Bacterial Diversity in Native Heart Valves in Infective Endocarditis.Biomedicines · 2025Article
- RpNGS: an automated platform for pathogen identification and monitoring in clinical metagenomics data.PeerJ · 2025Article
- Clinical application of metagenomic next-generation sequencing in the diagnosis of severe pneumonia pathogens.Frontiers in cellular and infection microbiology · 2025Article
- The diagnostic value of RNA-mNGS and DNA-mNGS in differentiating bacterial infection from colonization in the lower respiratory tract.Frontiers in cellular and infection microbiology · 2025Article
- Early diagnosis of streptococcus cristatus in blood culture-negative infective endocarditis by capture-based metagenomic next-generation sequencing: a case report.Frontiers in cardiovascular medicine · 2025Article
- Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2024Article
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- Diagnostic value of DNA or RNA-based metagenomic next-generation sequencing in lower respiratory tract infections.Heliyon · 2024Article
- Article
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11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objective: The present study aimed to prospectively evaluate the role of metagenomic next-generation sequencing (mNGS) in the etiological diagnosis of patients with perioperative infective endocarditis (IE). Methods: From May 1st, 2019 to December 31st, 2020, a total of 99 patients with IE were enrolled in the present study according to the modified Duke criteria, etiological, and pathological results. 11 non-IE patients undergoing heart valve surgery in the same period were selected as the control group. A blood culture test was performed immediately after admission, and the valves harvested operatively were examined by blood culture and mNGS. Results: In the IE group, there were 29 cases (29.3%) with positive blood culture, 16 cases (16.2%) with positive valve culture, and 85 cases (85.9%) with positive valve mNGS. Compared to culture-based detection, mNGS achieved better performance with a sensitivity, specificity, area under the curve (AUC) of 0.859, 0.727, and 0.793, respectively. The combined approach using culture and mNGS further improved the diagnostic accuracy (sensitivity 89.9%, specificity 72.7%, AUC 0.813). Preoperative white blood cell ( Conclusions: mNGS outperformed the conventional culture method and displayed high accuracy in detecting pathogens in IE patients. This study provided support for the use of mNGS in the etiological diagnosis of IE.
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