ReviewACS catalysis2022
Mechanism-Based Design of Efficient PET Hydrolases.
Review in ACS catalysis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
92 citing papers in PubMed, 1 synthesis or guideline pooled it, 257 citations in OpenAlex.
- The diversity of PET degrading enzymes: A systematic review of sequence, structure, and function.Protein science : a publication of the Protein Society · 2025Pooled it
- Deep Learning-driven synergistic engineering of PET hydrolase for post-consumer PET depolymerization.Synthetic and systems biotechnology · 2027Article
- A versatile Bacillus esterase reveals a carboxylesterase blueprint for PET intermediate degradation.Applied microbiology and biotechnology · 2026Article
- Advances in amidases and urethanases as depolymerization biocatalysts.Nature chemical biology · 2026Review
- Methods for the establishment of enzymatic mechanisms - from QM to ML.Chemical science · 2026Review
- Bimetallic Synergistic Catalysis Enabling Efficient Chemical Recycling of Commercial Polyesters.Polymer science & technology (Washington, D.C.) · 2026Article
- Experimental, Genomic, and Structural Evidence Supporting Putative PET-Hydrolases in Thermophilic Bacteria Isolated From Hot Springs in Cajamarca, Peru.MicrobiologyOpen · 2026Article
- Polyesterase activity and thermostability of carboxylesterases from Thermoleophilum album YS-3.The FEBS journal · 2026Article
- Temperature-Dependent Reorganization of Conformational Dynamics and Interaction Networks Underlies Thermostability in PET-Degrading Enzymes.International journal of molecular sciences · 2026Article
- Application of the YebF secretion pathway inRSC chemical biology · 2026Article
- Dynamics-informed multigraph neural networks for protein thermostability prediction and residue-level interpretation.iScience · 2026Article
- Enhancement of heat resistance ofEngineering microbiology · 2026Article
- Computational engineering of the polyester hydrolase PHL7 for efficient poly(ethylene terephthalate) degradation in biocatalytic recycling processes.Nature communications · 2026Article
- Carbon-Stimulated Bioaugmentation Enhances Thermogenesis, Lignocellulose Degradation, and Humification in Low-Temperature Cattle Manure Composting.Microorganisms · 2026Article
- Evolutionary engineering of Geobacillus thermoleovorans for growth on adipic acid and 1,4-butanediol.Applied microbiology and biotechnology · 2026Article
- Engineering a robust IsPETase for energy-efficient PET depolymerization in natural seawater at ambient temperatures.Advanced biotechnology · 2026Article
- Rethinking plastic waste: innovations in enzymatic breakdown of oil-based polyesters and bioplastics.FEBS open bio · 2026Review
- Toxic effects of microplastics in aquatic environments and the pathways to sustainable management.RSC advances · 2026Review
- Temperature-Dependent Active-Site Rearrangements of PETaseSM14: Insights from Molecular Dynamics Simulations.International journal of molecular sciences · 2026Article
- EngineeringiScience · 2026Article
32 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyethylene terephthalate (PET) is the most widespread synthetic polyester, having been utilized in textile fibers and packaging materials for beverages and food, contributing considerably to the global solid waste stream and environmental plastic pollution. While enzymatic PET recycling and upcycling have recently emerged as viable disposal methods for a circular plastic economy, only a handful of benchmark enzymes have been thoroughly described and subjected to protein engineering for improved properties over the last 16 years. By analyzing the specific material properties of PET and the reaction mechanisms in the context of interfacial biocatalysis, this Perspective identifies several limitations in current enzymatic PET degradation approaches. Unbalanced enzyme-substrate interactions, limited thermostability, and low catalytic efficiency at elevated reaction temperatures, and inhibition caused by oligomeric degradation intermediates still hamper industrial applications that require high catalytic efficiency. To overcome these limitations, successful protein engineering studies using innovative experimental and computational approaches have been published extensively in recent years in this thriving research field and are summarized and discussed in detail here. The acquired knowledge and experience will be applied in the near future to address plastic waste contributed by other mass-produced polymer types (e.g., polyamides and polyurethanes) that should also be properly disposed by biotechnological approaches.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.