Evidence map›Paper›PMID 35366889›Full record

ArticleJournal of nanobiotechnology2022

Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells.

Tong Lei, Yanyan Liu, Shiwen Deng, Zhuangzhuang Xiao, Yanjie Yang, Xiaoshuang Zhang, Wangyu Bi, Hongwu Du

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Designing lysate-based hydrogels for on-demand therapy.Journal of pharmaceutical analysis · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Tong Lei *School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, Lihua BLDG, No.30 Xueyuan Road, Haidian District, Beijing, 100083, China.
Yanyan Liu *Kangyanbao Stem Cell (Beijing) Co., Ltd, Beijing, 102629, China.
Shiwen DengDaxing Research Institute, University of Science and Technology Beijing, Beijing, 100083, China.
Zhuangzhuang XiaoSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, Lihua BLDG, No.30 Xueyuan Road, Haidian District, Beijing, 100083, China.
Yanjie YangSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, Lihua BLDG, No.30 Xueyuan Road, Haidian District, Beijing, 100083, China.
Xiaoshuang ZhangSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, Lihua BLDG, No.30 Xueyuan Road, Haidian District, Beijing, 100083, China.
Wangyu BiSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, Lihua BLDG, No.30 Xueyuan Road, Haidian District, Beijing, 100083, China.
Hongwu DuSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, Lihua BLDG, No.30 Xueyuan Road, Haidian District, Beijing, 100083, China. hongwudu@ustb.edu.cn.
University of Science and Technology Beijing · CNCell Technology (China) · CN

Funding

Hebei Provincial Department of Bureau of Science and Technology No.19942410G
6 · The paper itself

Abstract

Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients will increase the risk of zoonotic disease transmission. Human platelet lysate (HPL) is a potential surrogate and used in human cell expansion with reliability in clinical applications. In this study, we synthesized chitosan/gelatin/gellan gum hydrogel supplemented with HPL and investigated the effect of 3D culture for SHED. TMT-tagged proteomics was used to decipher the secretome protein profiles of SHEDs and a total of 3209 proteins were identified, of which 23 were up-regulated and 192 were down-regulated. The results showed that hydrogel supplemented with HPL promoted SHED proliferation. After induction, the hydrogel coating contributed to osteogenic differentiation, adipogenic differentiation and differentiation into neural-like cells of SHED. SHED encapsulated in a hydrogel promotes migration and angiogenesis of HUVEC. In conclusion, our research found that hydrogel supplemented with HPL can be used as a method for SHED in standardized production and can contribute to the clinical application of SHED in cell therapy.

Indexed as

Mesenchymal Stem CellsOsteogenesisCell DifferentiationHumansHydrogelsReproducibility of ResultsHydrogelsAngiogenesisHPLNeural-like cellsOsteogenesisSecretomeSHED

Identifiers

PMID35366889
PMCPMC8976277
OpenAlexW4226035629

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.