Evidence map›Paper›PMID 35366188›Full record

ArticleApplied biochemistry and biotechnology2022

Homologous Expression and Characterization of α-L-rhamnosidase from Aspergillus niger for the Transformation of Flavonoids.

Hangyu Ye, Xiaojun Li, Luyuan Li, Yinjun Zhang, Jianyong Zheng

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. An Organic Solvent-Tolerant α-L-Rhamnosidase fromMolecules (Basel, Switzerland) · 2025
    Article
  3. Article
  4. α-L-rhamnosidase: production, properties, and applications.World journal of microbiology & biotechnology · 2023
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Hangyu YeKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
Xiaojun LiDepartment of Fundamental Medicine, Xinyu University, Xinyu, 338004, People's Republic of China.
Luyuan LiKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
Yinjun ZhangKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
Jianyong ZhengKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China. zjy821212@zjut.edu.cn.ORCID http://orcid.org/0000-0002-9821-1062
Zhejiang University of Technology · CNXinyu University · CN

Funding

National Natural Science Foundation of China 31600639Science and Technology Project Founded by the Education Department of Jiangxi Province GJJ202305Zhejiang University Student Science and Technology Innovation Activity Plan 2021R403094
6 · The paper itself

Abstract

Aspergillus niger has been used for homologous and heterologous expressions of many protein products. In this study, the α-L-rhamnosidase from A. niger (Rha-N1, GenBank XP_001389086.1) was homologously expressed in A. niger 3.350 by Agrobacterium tumefaciens-mediated transformation. The enzyme activity of Rha-N1 was 0.658 U/mL, which was obtained by cultivation of engineered A. niger in a 5-L bioreactor. Rha-N1 was purified by affinity chromatography and characterized. The optimum temperature and optimum pH for Rha-N1 were 60 °C and 4.5, respectively. Enzyme activity was promoted by Al

Indexed as

Aspergillus nigerFlavonoidsAspergillusGlycoside HydrolasesRutinTemperaturealpha-L-rhamnosidaseFlavonoidsGlycoside HydrolasesRutinAgrobacterium tumefaciens-mediated transformationAspergillus nigerCharacterizationHomologous expressionα-L-rhamnosidase

Identifiers

PMID35366188
OpenAlexW4226109428

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.