ArticleNature communications2022
100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Using rotational integration of oblique interferometric scattering to track axial spatiotemporal responses of tubular membrane protrusions.Nature communications · 2026Article
- Models of cardiomyocyte-non-myocyte electrical interactions.The Journal of physiology · 2026Review
- Enhanced Interferometric Imaging by Rotating Coherent Scattering Microscopy.ACS photonics · 2025Article
- Review
- Roadmap on Label-Free Super-Resolution Imaging.Laser & photonics reviews · 2023Article
- Article
- Viewing life without labels under optical microscopes.Communications biology · 2023Review
- Label-free optical interferometric microscopy to characterize morphodynamics in living plants.Frontiers in plant science · 2023Review
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence techniques dominate the field of live-cell microscopy, but bleaching and motion blur from too long integration times limit dynamic investigations of small objects. High contrast, label-free life-cell imaging of thousands of acquisitions at 160 nm resolution and 100 Hz is possible by Rotating Coherent Scattering (ROCS) microscopy, where intensity speckle patterns from all azimuthal illumination directions are added up within 10 ms. In combination with fluorescence, we demonstrate the performance of improved Total Internal Reflection (TIR)-ROCS with variable illumination including timescale decomposition and activity mapping at five different examples: millisecond reorganization of macrophage actin cortex structures, fast degranulation and pore opening in mast cells, nanotube dynamics between cardiomyocytes and fibroblasts, thermal noise driven binding behavior of virus-sized particles at cells, and, bacterial lectin dynamics at the cortex of lung cells. Using analysis methods we present here, we decipher how motion blur hides cellular structures and how slow structure motions cover decisive fast motions.
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Registered trials
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