ArticleNature communications2022
Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- From Pits to Plaques: Electron Microscopy Reveals Clathrin Structural Plasticity in Trafficking, Sorting and Adhesion.Traffic (Copenhagen, Denmark) · 2026Review
- Protocol for detecting protein recruitment to clathrin structures in cultured cells.STAR protocols · 2026Article
- Clathrin is an Intrinsic Driver of Membrane Fission.bioRxiv : the preprint server for biology · 2026Article
- The Interplay of Lipids and Peptides in Membrane Merger.Advances in experimental medicine and biology · 2026Review
- Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosis.Nature communications · 2025Article
- Dynamic early recruitment of GAK-Hsc70 regulates coated pit maturation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells.Biophysical journal · 2025Article
- Lysosomal PIPProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Defining the Soluble and Extracellular Vesicle Protein Compartments of Plasma Using In-Depth Mass Spectrometry-Based Proteomics.Journal of proteome research · 2024Article
- Protein-membrane interactions: sensing and generating curvature.Trends in biochemical sciences · 2024Review
- Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells.bioRxiv : the preprint server for biology · 2024Article
- A live cell imaging-based assay for tracking particle uptake by clathrin-mediated endocytosis.Methods in enzymology · 2024Article
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
- DrSTAR: Tracking real-time nanometer axial changes.Biophysical journal · 2023Article
- Exocytosis of the silicified cell wall of diatoms involves extensive membrane disintegration.Nature communications · 2023Article
- Generation of nanoscopic membrane curvature for membrane trafficking.Nature reviews. Molecular cell biology · 2023Review
- Illuminating membrane structural dynamics of fusion and endocytosis with advanced light imaging techniques.Biochemical Society transactions · 2022Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Clathrin polymerization and changes in plasma membrane architecture are necessary steps in forming vesicles to internalize cargo during clathrin-mediated endocytosis (CME). Simultaneous analysis of clathrin dynamics and membrane structure is challenging due to the limited axial resolution of fluorescence microscopes and the heterogeneity of CME. This has fueled conflicting models of vesicle assembly and obscured the roles of flat clathrin assemblies. Here, using Simultaneous Two-wavelength Axial Ratiometry (STAR) microscopy, we bridge this critical knowledge gap by quantifying the nanoscale dynamics of clathrin-coat shape change during vesicle assembly. We find that de novo clathrin accumulations generate both flat and curved structures. High-throughput analysis reveals that the initiation of vesicle curvature does not directly correlate with clathrin accumulation. We show clathrin accumulation is preferentially simultaneous with curvature formation at shorter-lived clathrin-coated vesicles (CCVs), but favors a flat-to-curved transition at longer-lived CCVs. The broad spectrum of curvature initiation dynamics revealed by STAR microscopy supports multiple productive mechanisms of vesicle formation and advocates for the flexible model of CME.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.