ArticleStem cell research & therapy2022
Cell-free fat extract attenuates osteoarthritis via chondrocytes regeneration and macrophages immunomodulation.
Article in Stem cell research & therapy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Clinical efficacy of cell-free fat extract and its effects on bone marrow edema in patients with early to mid-stage knee osteoarthritis: a clinical trial in comparison with hyaluronic acid.Journal of orthopaedic surgery and research · 2025Trial
- Obesity-induced oleic acid metabolic dysregulation may exacerbate osteoarthritis through the degradation of SOX9.Cellular and molecular life sciences : CMLS · 2026Article
- Safety and Efficacy of Intra-articular Cell-Free Fat Extract Injection in Patients With Symptomatic Knee Osteoarthritis.Orthopaedic journal of sports medicine · 2026Article
- Cell-Free Fat Extract (CEFFE) as a Versatile Cell-Free Biologic for Multitissue Regeneration: From Skin and Bone to Nerve, Reproductive, and Systemic Applications-A Comprehensive Review.BioMed research international · 2026Review
- Recombinant Human Annexin A5 Ameliorates Localized Scleroderma by Inhibiting the Activation of Fibroblasts and Macrophages.Pharmaceutics · 2025Article
- Cell-Free Fat Extract (Ceffe) Combined with GelMA Hydrogel to Improve the Survival Rate of Random Skin Flaps in Mice.ACS biomaterials science & engineering · 2025Article
- Cell-Free Fat Extract for the Treatment of Lumbar Disc Degeneration: A Novel Approach Using Adipose-Derived Biologic.Biomedicines · 2025Article
- Cell-free fat extract prevents diminished ovarian reserve by inhibiting granulosa cell senescence.Stem cell research & therapy · 2025Article
- Adipose Tissue-Derived Therapies for Osteoarthritis: Multifaceted Mechanisms and Clinical Prospects.Cells · 2025Review
- Cell-Free Fat Extract with Anti-melanogenic Property Improves the Appearance of Infraorbital Dark Circles: A Preliminary Clinical Study.Aesthetic plastic surgery · 2025Article
- Cell-free adipose extract inhibits hypertrophic scar formation through collagen remodeling and antiangiogenesis.Open medicine (Warsaw, Poland) · 2025Article
- An engineered adipose formulation decreases hepatic inflammation and fibrosis in a rodent model of metabolic dysfunction-associated steatotic liver disease.Frontiers in bioengineering and biotechnology · 2025Article
- Recombinant human annexin A5 accelerates diabetic wounds healing by regulating skin inflammation.Regenerative therapy · 2024Article
- 3D printing of Ceffe-infused scaffolds for tailored nipple-like cartilage development.BMC biotechnology · 2024Article
- Cell-free fat extract promotes axon regeneration and retinal ganglion cells survival in traumatic optic neuropathy.Frontiers in cellular neuroscience · 2024Article
- Annexin A5 Derived from Cell-free Fat Extract Attenuates Osteoarthritis via Macrophage Regulation.International journal of biological sciences · 2024Article
- Cell-free fat extract restores hair loss: a novel therapeutic strategy for androgenetic alopecia.Stem cell research & therapy · 2023Article
- Metabolic Mechanisms and Potential Therapeutic Targets for Prevention of Ovarian Aging: Data from Up-to-Date Experimental Studies.International journal of molecular sciences · 2023Review
- Nrf2-mediated anti-inflammatory polarization of macrophages as therapeutic targets for osteoarthritis.Frontiers in immunology · 2022Review
- Regeneration of articular cartilage defects: Therapeutic strategies and perspectives.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe prevalence of osteoarthritis (OA) is increasing, yet clinically effective and economical treatments are unavailable. We have previously proposed a cell-free fat extract (CEFFE) containing multiple cytokines, which possessed antiapoptotic, anti-oxidative, and proliferation promotion functions, as a "cell-free" strategy. In this study, we aimed to evaluate the therapeutic effect of CEFFE in vivo and in vitro.
methodsIn vivo study, sodium iodoacetate-induced OA rats were treated with CEFFE by intra-articular injections for 8 weeks. Behavioral experiments were performed every two weeks. Histological analyses, anti-type II collagen, and toluidine staining provided structural evaluation. Macrophage infiltration was assessed by anti-CD68 and anti-CD206 staining. In vitro study, the effect of CEFFE on macrophage polarization and secretory factors was evaluated by flow cytometry, immunofluorescence, and quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The effect of CEFFE on cartilage regeneration was accessed by cell counting kit-8 assay and qRT-PCR. The generation of reactive oxygen species (ROS) and levels of ROS-related enzymes were investigated by qRT-PCR and western blotting.
resultsIn rat models with sodium iodoacetate (MIA)-induced OA, CEFFE increased claw retraction pressure while decreasing bipedal pressure in a dose-dependent manner. Moreover, CEFFE promoted cartilage structure restoration and increased the proportion of CD206
conclusionsCEFFE inhibits the progression of OA by promoting cartilage regeneration and limiting low-grade joint inflammation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.